Core promoter sequences contribute to ovo-B regulation in the Drosophila melanogaster germline

Genetics
Beata BielinskaB Oliver

Abstract

Utilization of tightly linked ovo-A vs. ovo-B germline promoters results in the expression of OVO-A and OVO-B, C(2)H(2) transcription factors with different N -termini, and different effects on target gene transcription and on female germline development. We show that two sex-determination signals, the X chromosome number within the germ cells and a female soma, differentially regulate ovo-B and ovo-A. We have previously shown that OVO regulates ovarian tumor transcription by binding the transcription start site. We have explored the regulation of the ovo-B promoter using an extensive series of transgenic reporter gene constructs to delimit cis-regulatory sequences as assayed in wild-type and sex-transformed flies and flies with altered ovo dose. Minimum regulated expression of ovo-B requires a short region flanking the transcription start site, suggesting that the ovo-B core promoter bears regulatory information in addition to a "basal" activity. In support of this idea, the core promoter region binds distinct factors in ovary and testis extracts, but not in soma extracts, suggesting that regulatory complexes form at the start site. This idea is further supported by the evolutionarily conserved organization of OVO binding site...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 1, 1987·Genes & Development·B OliverA P Mahowald
Jun 20, 1998·Development Genes and Evolution·J LüB Oliver
Sep 5, 2002·International Review of Cytology·Brian Oliver
Jan 10, 2003·Nucleic Acids Research·UNKNOWN FlyBase Consortium
May 23, 2003·Journal of Biology·Boris LenhardWyeth W Wasserman
Sep 2, 2003·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 23, 2003·Nucleic Acids Research·Oliver D King, Frederick P Roth
Dec 19, 2003·Nucleic Acids Research·Dennis A BensonDavid L Wheeler
Jul 17, 2004·Genome Research·Peter C FitzGeraldCharles Vinson

❮ Previous
Next ❯

Citations

Jul 11, 2006·Genome Biology·Peter C FitzGeraldCharles Vinson
Mar 2, 2006·Journal of Biology·Vaijayanti GuptaBrian Oliver
Jun 2, 2005·Developmental Biology·Toshie KaiAllan C Spradling
Jan 7, 2017·Scientific Reports·Makoto HayashiSatoru Kobayashi
Mar 5, 2015·The FEBS Journal·András HorváthBeáta G Vértessy
Dec 5, 2019·Communications Biology·Shoichi NakamuraMasanori Mukai
Jan 12, 2020·Communications Biology·Shoichi NakamuraMasanori Mukai
Jul 13, 2006·Development·Abbie Casper, Mark Van Doren
Jul 6, 2014·Genome Research·Zhen-Xia ChenStephen Richards

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.