Core promoter-specific gene regulation: TATA box selectivity and Initiator-dependent bi-directionality of serum response factor-activated transcription

Biochimica Et Biophysica Acta
Muyu XuErnest Martinez

Abstract

Gene-specific activation by enhancers involves their communication with the basal RNA polymerase II transcription machinery at the core promoter. Core promoters are diverse and may contain a variety of sequence elements such as the TATA box, the Initiator (INR), and the downstream promoter element (DPE) recognized, respectively, by the TATA-binding protein (TBP) and TBP-associated factors of the TFIID complex. Core promoter elements contribute to the gene selectivity of enhancers, and INR/DPE-specific enhancers and activators have been identified. Here, we identify a TATA box-selective activating sequence upstream of the human β-actin (ACTB) gene that mediates serum response factor (SRF)-induced transcription from TATA-dependent but not INR-dependent promoters and requires the TATA-binding/bending activity of TBP, which is otherwise dispensable for transcription from a TATA-less promoter. The SRF-dependent ACTB sequence is stereospecific on TATA promoters but activates in an orientation-independent manner a composite TATA/INR-containing promoter. More generally, we show that SRF-regulated genes of the actin/cytoskeleton/contractile family tend to have a TATA box. These results suggest distinct TATA-dependent and INR-dependent m...Continue Reading

References

Dec 25, 1991·Nucleic Acids Research·S L DanilitionN G Miyamoto
Jan 25, 1989·Nucleic Acids Research·S Y NgL Kedes
Nov 1, 1995·Molecular and Cellular Biology·K H EmamiS T Smale
Dec 1, 1995·Molecular and Cellular Biology·V Iyer, K Struhl
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·E MartinezR G Roeder
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·I P GarrawayS T Smale
May 15, 1996·Genes & Development·C MerliR K Blackman
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Nov 1, 1996·Molecular and Cellular Biology·P ErnstS T Smale
Oct 3, 2001·Genes & Development·J E Butler, J T Kadonaga
Jan 9, 2003·Plant Molecular Biology·Ernest Martinez
Mar 26, 2003·Annual Review of Biochemistry·Stephen T Smale, James T Kadonaga
Apr 29, 2004·Nature Structural & Molecular Biology·Steven Hahn
Oct 19, 2005·Genes & Development·Wensheng Deng, Stefan G E Roberts
Aug 25, 2006·American Journal of Physiology. Cell Physiology·Joseph M MianoKeigi Fujiwara
Jan 12, 2008·Nucleic Acids Research·Paolo BenattiCarol Imbriano
Jan 24, 2008·Molecular and Cellular Biology·Michele CeribelliRoberto Mantovani
Apr 26, 2008·Current Opinion in Cell Biology·Tamar Juven-GershonJames T Kadonaga
Oct 17, 2008·Genes & Development·Tamar Juven-GershonJames T Kadonaga
Aug 18, 2009·Developmental Biology·Tamar Juven-Gershon, James T Kadonaga
Dec 1, 2009·Cell Cycle·Diletta DolfiniRoberto Mantovani
Jan 26, 2010·European Journal of Cell Biology·Daniela WerthOlaf Heidenreich

❮ Previous
Next ❯

Citations

Apr 27, 2019·Frontiers in Genetics·Mhaned OubounytKil To Chong
Nov 30, 2020·Cytoskeleton·Bieke VanslembrouckJolanda van Hengel
May 26, 2020·Mechanisms of Development·Christopher D Clark, Kyu-Ho Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.