Core protein cleavage by signal peptide peptidase is required for hepatitis C virus-like particle assembly

The Journal of General Virology
Malika Ait-GoughoultePhilippe Roingeard

Abstract

Hepatitis C virus (HCV) core protein, expressed with a Semliki Forest virus replicon, self-assembles into HCV-like particles (HCV-LP) at the endoplasmic reticulum (ER) membrane, providing an opportunity to study HCV assembly and morphogenesis by electron microscopy. This model was used to investigate whether the processing of the HCV core protein by the signal peptide peptidase (SPP) is required for the HCV-LP assembly. Several mutants were designed as there are conflicting reports concerning the cleavage of mutant proteins by SPP. Production of the only core mutant protein that escaped SPP processing led to the formation of multiple layers of electron-dense ER membrane, with no evidence of HCV-LP assembly. These data shed light on the HCV core residues involved in SPP cleavage and suggest that this cleavage is essential for HCV assembly.

References

Jun 17, 1998·Journal of Virology·K YasuiM Kohara
Mar 22, 2002·Journal of Virology·Emmanuelle BlanchardPhilippe Roingeard
Jun 12, 2002·Biochemical and Biophysical Research Communications·Takayuki YamanakaTakefumi Doi
Jun 22, 2002·Science·Andreas WeihofenBruno Martoglio
Nov 7, 2002·Molecular Cell·Marius K Lemberg, Bruno Martoglio
Mar 7, 2003·The Journal of Biological Chemistry·Andreas WeihofenBruno Martoglio
Apr 8, 2003·Nature Cell Biology·Dieter EdbauerChristian Haass
Aug 28, 2003·Journal of Virology·Emmanuelle BlanchardPhilippe Roingeard
May 1, 2004·Biology of the Cell·Philippe RoingeardMalika Ait-Goughoulte
May 28, 2004·Journal of Virology·Kiyoko OkamotoYoshiharu Matsuura
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jin ZhongFrancis V Chisari
Jun 11, 2005·Science·Brett D LindenbachCharles M Rice

❮ Previous
Next ❯

Citations

Feb 2, 2008·Cellular and Molecular Life Sciences : CMLS·A J Beel, C R Sanders
May 31, 2008·Histochemistry and Cell Biology·Philippe RoingeardGérard Prensier
Apr 17, 2009·Journal of Virology·Maria E PicconeStanley N Cohen
Feb 3, 2007·Retrovirology·Anne GatignolJean-Luc Darlix
Apr 28, 2010·Cellular and Molecular Life Sciences : CMLS·Marion DeplaPhilippe Roingeard
Jun 27, 2009·Pharmacology & Therapeutics·Claire GondeauPatrick Maurel
May 12, 2009·Seminars in Cell & Developmental Biology·Todd E GoldeDoron C Greenbaum
Jan 27, 2009·Biochimica Et Biophysica Acta·John McLauchlan
Dec 19, 2007·Journal of Viral Hepatitis·P Roingeard, C Hourioux
Sep 11, 2007·The FEBS Journal·Morgane RégeardJacques Le Seyec
Jan 9, 2008·Journal of Viral Hepatitis·A J Pérez-BernáJ Villalaín
Jul 17, 2008·Biology of the Cell·Philippe Roingeard
Apr 5, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Saba KhaliqSajida Hassan
Jun 12, 2009·Clinical Science·Hengli Tang, Henry Grisé
Feb 5, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Saba KhaliqAsim Pervaiz
Jan 5, 2011·Biochimica Et Biophysica Acta·M Francisca Palomares-Jerez, José Villalaín
Jul 28, 2009·Biochimica Et Biophysica Acta·Jaime GuillénJosé Villalaín
Nov 28, 2016·The FEBS Journal·Steven H L Verhelst
Jun 19, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Torben MentrupBernd Schröder
Sep 16, 2016·Journal of Virology·Karen BayerMichael Schindler
Feb 15, 2018·Virology Journal·Ana Carolina Gomes JardimMark Harris
Jan 1, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Hossein KeyvaniHamid Reza Mollaie
Mar 13, 2018·World Journal of Hepatology·Vladimir Alexei Morozov, Sylvie Lagaye
Aug 3, 2006·The Journal of Biological Chemistry·Paul Targett-AdamsJohn McLauchlan
Jan 17, 2008·The Journal of Biological Chemistry·Ana J Pérez-BernáJosé Villalaín
Apr 22, 2008·The Journal of Biological Chemistry·Paul Targett-AdamsJohn McLauchlan
Feb 14, 2020·Cellular and Molecular Life Sciences : CMLS·Torben MentrupBernd Schröder
May 16, 2007·Médecine sciences : M/S·Philippe Roingeard, Christophe Hourioux

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.