Core-shell polymeric nanoparticles comprising BODIPY and fluorescein as ultra-bright ratiometric fluorescent pH sensors

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Chloé GrazonGilles Clavier

Abstract

A new ratiometric fluorescent pH nanosensor is presented. It is based on ultrabright nanoparticles containing two spatially separated fluorophores: BODIPY covalently linked to the polystyrene core and fluorescein grafted to the nanoparticle shell. The nanoparticles comprise a large number (≥2500) of both fluorescent moieties. Their spectroscopic characteristics were studied at different pH and ionic strength. They could successfully be used to determine the solution pH between 5.5 and 7.5 by measuring the fluorescence intensity ratio of the sensor molecule (fluorescein) relative to the reference dye (BODIPY).

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Citations

Feb 23, 2020·Photochemistry and Photobiology·Ruth Prieto-MonteroVirginia Martínez-Martínez

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Methods Mentioned

BETA
FRET
size exclusion chromatography
dynamic light scattering
transmission electron microscopy
NMR
Fluorescence

Software Mentioned

Viscotek OmniSEC
iTEM

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