Apr 5, 2020

SARS-CoV-2 and SARS-CoV differ in their cell tropism and drug sensitivity profiles

BioRxiv : the Preprint Server for Biology
D. BojkovaJ. N. Cinatl

Abstract

SARS-CoV-2 is a novel coronavirus currently causing a pandemic. We show that the majority of amino acid positions, which differ between SARS-CoV-2 and the closely related SARS-CoV, are differentially conserved suggesting differences in biological behaviour. In agreement, novel cell culture models revealed differences between the tropism of SARS-CoV-2 and SARS-CoV. Moreover, cellular ACE2 (SARS-CoV-2 receptor) and TMPRSS2 (enables virus entry via S protein cleavage) levels did not reliably indicate cell susceptibility to SARS-CoV-2. SARS-CoV-2 and SARS-CoV further differed in their drug sensitivity profiles. Thus, only drug testing using SARS-CoV-2 reliably identifies therapy candidates. Therapeutic concentrations of the approved protease inhibitor aprotinin displayed anti-SARS-CoV-2 activity. The efficacy of aprotinin and of remdesivir (currently under clinical investigation against SARS-CoV-2) were further enhanced by therapeutic concentrations of the proton pump inhibitor omeprazole (aprotinin 2.7-fold, remdesivir 10-fold). Hence, our study has also identified anti-SARS-CoV-2 therapy candidates that can be readily tested in patients.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Genome
Somatic Mutation
Cancer Gene Mutation
Retrotransposons
Basal Cell Neoplasm
Nucleotides
COG1 gene
Base Sequence
Simulation
Analysis

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

American Journal of Clinical Pathology
Ahmed BabikerJeannette Guarner
The New England Journal of Medicine
Xiaoxia LuChinese Pediatric Novel Coronavirus Study Team
Journal of Medical Virology
Beuy Joob, Viroj Wiwanitkit
BioRxiv : the Preprint Server for Biology
Kumari G LokugamageVineet D Menachery
© 2020 Meta ULC. All rights reserved