Core/Shell Gene Carriers with Different Lengths of PLGA Chains to Transfect Endothelial Cells

Langmuir : the ACS Journal of Surfaces and Colloids
Xinghong DuoWencheng Zhang

Abstract

In order to improve the transfection efficiency and reduce the cytotoxicity of gene carriers, many strategies have been used to develop novel gene carriers. In this study, five complex micelles (MSP(2 k), MSP(4 k), MSP(6 k), MSP(8 k), and MSP(10 k)) were prepared from methoxy-poly(ethylene glycol)-b-poly(d,l-lactide-co-glycolide) (mPEG-b-PLGA) and sorbitol-poly(d,l-lactide-co-glycolide)-graft-PEI (sorbitol-PLGA-g-PEI, where the designed molecular weights of PLGA chains were 2 kDa, 4 kDa, 6 kDa, 8 kDa, and 10 kDa, respectively) copolymers by a self-assembly method, and the mass ratio of mPEG-b-PLGA to sorbitol-PLGA-g-PEI was 1/3. These complex micelles and their gene complexes had appropriate sizes and zeta potentials, and pEGFP-ZNF580 (pDNA) could be efficiently internalized into EA.hy926 cells by their gene complexes (MSP(2 k)/pDNA, MSP(4 k)/pDNA, MSP(6 k)/pDNA, MSP(8 k)/pDNA, and MSP(10 k)/pDNA). The MTT assay results demonstrated that the gene complexes had low cytotoxicity in vitro. When the hydrophobic PLGA chain increased above 6 kDa, the gene complexes showed higher performance than that prepared from short hydrophobic chains. Moreover, the relative ZNF580 protein expression levels in MSP(6 k)/pDNA, MSP(8 k)/pDNA, and MS...Continue Reading

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Citations

Oct 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xuefang HaoYakai Feng
Mar 17, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Mandy H M Leung, Amy Q Shen
Aug 19, 2020·ACS Applied Materials & Interfaces·Lingchuang BaiJiandong Ding
Oct 8, 2018·ACS Biomaterials Science & Engineering·Xiangyu LiangJiandong Ding

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