Corium tissue expression of genes associated with inflammation, oxidative stress, and keratin formation in relation to lameness in dairy cows

Journal of Dairy Science
J S OsorioJuan J Loor


Objectives were to (1) determine the feasibility of performing hoof biopsies without impairing locomotion; (2) evaluate the feasibility of using biopsied tissue for quantitative PCR; and (3) compare relative gene expression among claws for several target genes. Biopsies were performed on 6 Holstein cows, yielding 4 tissue specimens per cow from front leg, right limb, and medial claw (claw position 3); rear leg, left limb, and lateral claw (claw position 5); and rear leg, right limb, medial claw (claw position 7). Cows were monitored for lameness daily for 7 d post-biopsy and then weekly for 8 wk. Histopathological analysis confirmed that tissue collected was from between the stratum corneum and dermis. Biopsied tissue was used for RNA extraction, including evaluation of yield and purity. The profile by claw position of 19 genes with key functions in cell differentiation, proliferation, inflammation, and keratin formation was assessed via quantitative reverse transcription-PCR. Other than transient disturbances in locomotion score in some cows during 2 to 4 d post-biopsy, no signs of pain, locomotion impairment, or clinical lameness were observed post-biopsy. Total RNA yields averaged 259.7±100, 447.8±288, and 496.4±118 μg/mg of...Continue Reading


Feb 1, 1994·Journal of Comparative Pathology·S S SinghW R Ward
Aug 21, 1993·The Veterinary Record·S S SinghR D Murray
Jul 1, 1999·Anatomia, histologia, embryologia·C H MüllingW Steinberg
Feb 17, 2001·Applied Animal Behaviour Science·F C Flower, D M. Weary
Apr 4, 2002·The Journal of Dairy Research·K A HendryC J Wilde
Jul 31, 2002·Journal of Dairy Science·P P J van der TolW A Weijs
Jan 27, 2004·Journal of Dairy Science·Christer BergstenC C Gay
Dec 17, 2005·Journal of Dairy Science·F C Flower, D M Weary
Jun 15, 2006·Journal of Dairy Science·S NeveuxA M de Passillé
Apr 19, 2008·Journal of Dairy Science·N CapionC Enevoldsen
Jul 22, 2008·The Veterinary Record·N CapionC Enevoldsen
Aug 2, 2008·BMC Genomics·Massimo Bionaz, Juan J Loor
Jan 24, 2009·Journal of Dairy Science·A M DanscherM B Thoefner
May 9, 2009·Journal of Mammary Gland Biology and Neoplasia·Sara PensaValeria Poli
Jun 6, 2009·American Journal of Veterinary Research·Brenna K HanlyRustin M Moore
Jul 10, 2009·The Journal of Clinical Investigation·M Bishr OmaryShinichiro Hanada
Dec 26, 2009·Journal of Comparative Pathology·A M DanscherO L Nielsen
Jan 12, 2010·Journal of Dairy Science·P P J van der TolB Aernouts
Dec 15, 2010·Equine Veterinary Journal·Britta S LeiseJames K Belknap
Jan 26, 2011·The International Journal of Biochemistry & Cell Biology·Ching-Fang HsuHung-Shu Chang
May 17, 2011·The Veterinary Journal·E MuggliK Nuss
Sep 6, 2011·Veterinary Immunology and Immunopathology·Rafael R FaleirosJames K Belknap

Related Concepts

Bos indicus
Cattle Diseases
Foot Diseases
Keratin-Associated Proteins
Lameness, Animal

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.