Corium tissue expression of genes associated with inflammation, oxidative stress, and keratin formation in relation to lameness in dairy cows

Journal of Dairy Science
J S OsorioJuan J Loor

Abstract

Objectives were to (1) determine the feasibility of performing hoof biopsies without impairing locomotion; (2) evaluate the feasibility of using biopsied tissue for quantitative PCR; and (3) compare relative gene expression among claws for several target genes. Biopsies were performed on 6 Holstein cows, yielding 4 tissue specimens per cow from front leg, right limb, and medial claw (claw position 3); rear leg, left limb, and lateral claw (claw position 5); and rear leg, right limb, medial claw (claw position 7). Cows were monitored for lameness daily for 7 d post-biopsy and then weekly for 8 wk. Histopathological analysis confirmed that tissue collected was from between the stratum corneum and dermis. Biopsied tissue was used for RNA extraction, including evaluation of yield and purity. The profile by claw position of 19 genes with key functions in cell differentiation, proliferation, inflammation, and keratin formation was assessed via quantitative reverse transcription-PCR. Other than transient disturbances in locomotion score in some cows during 2 to 4 d post-biopsy, no signs of pain, locomotion impairment, or clinical lameness were observed post-biopsy. Total RNA yields averaged 259.7±100, 447.8±288, and 496.4±118 μg/mg of...Continue Reading

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Related Concepts

Biopsy
Bos indicus
Cattle Diseases
Foot Diseases
Cistron
Hoof
Inflammation
Keratin-Associated Proteins
Lameness, Animal
Locomotion

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