Corneal abnormalities in the NC/Nga mouse: an atopic dermatitis model

Cornea
Nobuyuki EbiharaChisei Ra

Abstract

To study corneal abnormalities in the NC/Nga mouse, which is an animal model of atopic dermatitis. To study histopathologic changes of the eyelid, conjunctiva, and cornea, we extracted the eyeballs together with upper and lower eyelids and fixed them for examination by light and electron microscopy or snap-froze them for immunohistochemistry. Transferase mediated-dUTP digoxigenin nick-end labeling staining was performed to detect apoptotic cells. In order to assess eye scratching behavior and the effect of tacroliums hydrate ointment, we made video recordings. Mice kept in a conventional room suffered from various grades of blepharoconjunctivitis and scratched their eyes furiously. Tacrolimus hydrate ointment reduced their eye-scratching behavior. Histopathologic study of the eyelid and conjunctiva showed that this blepharoconjunctivitis was caused by allergic inflammation. Mice with severe blepharoconjunctivitis showed thinning of the corneal epithelium, an irregular interface between the epithelium and stroma, subepithelial deposition of materials, and neovascularization of the stroma. Their corneas were cone shaped. Many transferase mediated-dUTP digoxigenin nick-end labeling-positive cells were recognized among superficial ...Continue Reading

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Citations

Mar 13, 2012·Cornea·Joel Sugar, Marian S Macsai
Mar 24, 2012·Allergology International : Official Journal of the Japanese Society of Allergology·Yusuke HaraMitsuru Sawa
Aug 16, 2011·Trends in Neurosciences·Andrew D Huberman, Cristopher M Niell
Sep 19, 2016·American Journal of Ophthalmology·Roberto CaputoBennie H Jeng
Sep 2, 2020·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·Elisabeth M Messmer
Jun 12, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Adrien MazharianDamien Gatinel
Feb 1, 2011·Current Eye Research·Kaoru KarasawaHiroshi Matsuda
May 23, 2014·Investigative Ophthalmology & Visual Science·Matthew J TurnerYang Sun
Nov 12, 2010·Ophthalmic Genetics·Loic PinsardJoseph Colin

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