Corneal Collagen Cross-Linking With Riboflavin and UVA Regulates Hemangiogenesis and Lymphangiogenesis in Rats

Investigative Ophthalmology & Visual Science
Yirui ZhuWei Chen

Abstract

The purpose of this study was to determine whether corneal collagen crosslinking (CXL) inhibits hemangiogenesis and lymphangiogenesis during acute corneal inflammation in an in vivo rat model. Inflammatory corneal neovascularization was induced by suture placement into a rat cornea. At day 3 after suture, a CXL protocol using riboflavin and UVA was administered after mechanical epithelial debridement. Hemangiogenesis and lymphangiogenesis were analyzed morphometrically. CD45 and CD68 immunostaining evaluated corneal leucocyte and macrophage immune cell infiltration, respectively. A TUNEL assay detected stromal cell apoptosis. Quantitative RT-PCR analysis identified angiogenic and lymphangiogenic genes as well as proinflammatory cytokine expression. Western blot analysis characterized vascular endothelial cell CD31 and lymphatic vessel endothelial hyaluronan receptor (LYVE-1) protein expression. CXL treatment significantly reduced corneal pathologic suture-induced hemangiogenesis and lymphangiogenesis 7 days after suture emplacement, but this procedure failed to affect hemangiogenesis and lymphangiogenesis 14 days after suture. Increased cell apoptosis and reduced CD45+ and CD68+ cell infiltration were evident in CXL-treated rat...Continue Reading

References

Feb 26, 1999·The Journal of Cell Biology·S BanerjiD G Jackson
Aug 17, 2001·Current Opinion in Ophthalmology·J H ChangD T Azar
Jan 31, 2003·Investigative Ophthalmology & Visual Science·Balamurali K AmbatiJayakrishna Ambati
Oct 3, 2003·Journal of Cataract and Refractive Surgery·Gregor WollensakTheo Seiler
Apr 23, 2004·Ophthalmic Research·Yumiko TakamiTsugio Amemiya
Nov 26, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·David G Jackson
Sep 12, 2006·European Journal of Pharmacology·Caryne M BertolloMárcio M Coelho
Sep 25, 2008·Acta Ophthalmologica·Shiqi LingZuguo Liu
Feb 10, 2009·Current Pharmaceutical Design·Zhao Ming David DongRoberto F Nicosia
Oct 3, 2009·Investigative Ophthalmology & Visual Science·Beatrice Bourghardt PeeboNeil Lagali
Dec 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tina DietrichClaus Cursiefen
Jan 27, 2010·Progress in Retinal and Eye Research·David EllenbergJin-Hong Chang
Feb 11, 2012·Investigative Ophthalmology & Visual Science·Yun HanZuguo Liu
Jan 26, 2013·Progress in Retinal and Eye Research·Felix BockClaus Cursiefen
Feb 15, 2013·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Keith M Meek, Sally Hayes
Apr 13, 2013·Investigative Ophthalmology & Visual Science·Benoît DetryAgnès Noël
Aug 24, 2013·Investigative Ophthalmology & Visual Science·Wei ChenQian Garrett
Nov 26, 2014·Environmental and Molecular Mutagenesis·Eva SedlačkováAlena Gábelová
Sep 9, 2015·Investigative Ophthalmology & Visual Science·Chang Rae RhoChoun-Ki Joo
Dec 26, 2015·Journal of Cataract and Refractive Surgery·Antonio MoramarcoLuigi Fontana
Jun 22, 2016·DNA Repair·Peter Karran, Reto Brem
Jun 23, 2016·Scientific Reports·Yanfeng ChenZuguo Liu
Apr 20, 2018·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Yanhong HouClaus Cursiefen

❮ Previous
Next ❯

Citations

Aug 17, 2021·Journal of Current Glaucoma Practice·Zeynep AktasAtike B Tefon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved