PMID: 8949720Aug 1, 1996Paper

Corneal organ culture: effects of serum and a stabilised form of L-glutamine

The British Journal of Ophthalmology
M G AyoubiD L Easty

Abstract

Organ culture medium for corneas contains labile components, such as L-glutamine, whose loss could be a limiting factor to the length of storage. The medium is also supplemented with fetal bovine serum (FBS), which can vary significantly between different batches. The aim of this study was to establish the need for FBS during corneal organ culture, and to determine whether substitution of L-glutamine by the stable dipeptide L-analyl-L-glutamine was beneficial. Porcine corneoscleral discs were suspended in 80 ml of organ culture medium (HEPES buffered Eagle's MEM with Earle's salts, 26 mmol/l NaHCO3, penicillin, streptomycin, and amphotericin B) and kept at 34 degrees C. The medium contained either 2 mmol/l L-glutamine or 2 mmol/l L-analyl-L-glutamine, and was either serum free or contained 2% FBS. At weekly intervals, five corneas from each group were stained with trypan blue and alizarin red S, and the surface area and shape of 100 endothelial cells were determined for each cornea. No differences were observed between corneas in organ culture medium with L-glutamine or L-analyl-L-glutamine. In serum free medium, endothelial cell density remained constant for the first week, but then declined rapidly over the next 2 weeks. With...Continue Reading

References

Dec 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·G Wu, L W Greene
Aug 1, 1992·The British Journal of Ophthalmology·R M RedmondD L Easty
Nov 1, 1990·The British Journal of Ophthalmology·W J ArmitageB A Bradley
Jun 1, 1989·Acta Ophthalmologica·J O HjortdalC U Andersen
Jan 1, 1981·Journal of Perinatal Medicine·R Terinde

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Citations

Mar 27, 2003·Cornea·Davide CamposampieroDiego Ponzin
Aug 25, 2001·The British Journal of Ophthalmology·T Møller-PedersenK Engelmann
Jun 22, 2017·Acta Ophthalmologica·Mohit ParekhAlessandro Ruzza
Apr 13, 2018·Current Eye Research·Ingrida SmeringaiovaKaterina Jirsova
May 11, 2012·Ophthalmic Research·M ZhaoP Gain
Jan 1, 2004·European Journal of Ophthalmology·D CamposampieroD Ponzin

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