Coronary reperfusion following ischemia: different expression of bcl-2 and bax proteins, and cardiomyocyte apoptosis

Japanese Heart Journal
Z XieK Nagashima

Abstract

The aim of this work was to examine factors that could be involved in the occurrence of apoptosis in rat hearts subjected to coronary occlusion followed by reperfusion. To this end, we studied the expression of the pro- and anti-apoptotic factors, bax and bcl-2, respectively, in reperfused ischemic hearts and in hearts injected with bFGF or saline. In anesthetized rats the left coronary artery was occluded for 45 min, the anesthesia withdrawn and the occlusion removed to allow reperfusion; in sham-operated rats the occlusion was omitted. After 4 hours the rats were decapitated and the heart excised. Sections from the left ventricle were stained with anti-bcl-2-antibody and anti-bax-antibody using the TUNEL method which detects apoptosis. Fragmentation of DNA isolated from reperfused ventricles was examined by agarose electrophoresis. In reperfused hearts no bcl-2 staining was observed in the discrete area in which many cardiomyocyte nuclei were stained by the TUNEL method; outside this area staining for bcl-2 was more marked than in sham-operated rats. Sections from reperfused hearts were stained for bax protein over a wide area including the apoptotic region; sham-operated hearts showed little reaction. Staining for bcl-2 was ...Continue Reading

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Citations

Sep 17, 2003·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Hesong ZengHuayue Zhao
Apr 23, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ying LiuDong-Sheng Pei
May 29, 2014·Drug Design, Development and Therapy·Nan WuDalin Jia
Oct 27, 2015·International Journal of Molecular Medicine·Jiajia YanMiaomiao Xi
Sep 16, 2008·Biochemical and Biophysical Research Communications·Rui-Zhen Shi, Qing-Ping Li
Dec 26, 2006·Journal of Molecular and Cellular Cardiology·Meifeng XuMuhammad Ashraf
Nov 9, 2004·Biochemical and Biophysical Research Communications·Wenyuan ZhaoYao Sun
Apr 25, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Zu-An ZhuYing Liu
Jul 15, 2016·Chinese Journal of Integrative Medicine·Li-Na MaYi-Kui Li
Jan 24, 2006·Journal of Molecular and Cellular Cardiology·David C ZebrowskiJunichi Sadoshima
Jan 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Pei-Hua LiDong-Sheng Pei
Dec 5, 2017·Experimental and Therapeutic Medicine·Guan-Lin YangNi Zhang
Jan 11, 2021·Molecular and Cellular Biochemistry·Rajshekhar A KoreJawahar L Mehta

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