Coronary vascular and endothelial reactivity changes in transgenic mice overexpressing atrial natriuretic factor

The American Journal of Physiology
D D KuM E Steinhelper

Abstract

Recent advances in genetic methods permit the introduction of random and defined mutations into the mouse germ line, producing novel mouse strains, some of which affect the heart and vasculature. A TTR-ANF transgenic strain of mice, which constitutively expresses a fusion gene consisting of the transthyretin promoter and the ANF structural gene, has been shown to result in a lifelong elevation of plasma atrial natriuretic factor (ANF) and a chronically lowered arterial blood pressure. However, no established method for efficient functional analysis of possible alterations in coronary vascular function in mice has been reported. In the present study, we describe an isolated mouse coronary artery preparation that permits an effective and reproducible evaluation of coronary endothelial and vascular function. Both left main and right coronary arteries (resting luminal diam 70-90 microns) were isolated and pressurized, and changes in luminal diameter were determined by videomicroscopy. The coronary pressure-luminal diameter relationship was not significantly different between TTR-ANF transgenic and nontransgenic littermates. Relaxation of coronaries to 0.1-100 nM ANF was significantly reduced in transgenic [maximum effect (Emax) = 4...Continue Reading

Citations

Dec 19, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Robert GrosMansoor Husain
Dec 7, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Xiaowei Sun, David D Ku
Feb 22, 2001·Annual Review of Physiology·B Wedel, D Garbers
Sep 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Xiaowei Sun, David D Ku
May 30, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Xiaowei Sun, David D Ku

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