Coronavirus particle assembly: primary structure requirements of the membrane protein

Journal of Virology
C A de HaanPeter J M Rottier

Abstract

Coronavirus-like particles morphologically similar to normal virions are assembled when genes encoding the viral membrane proteins M and E are coexpressed in eukaryotic cells. Using this envelope assembly assay, we have studied the primary sequence requirements for particle formation of the mouse hepatitis virus (MHV) M protein, the major protein of the coronavirion membrane. Our results show that each of the different domains of the protein is important. Mutations (deletions, insertions, point mutations) in the luminal domain, the transmembrane domains, the amphiphilic domain, or the carboxy-terminal domain had effects on the assembly of M into enveloped particles. Strikingly, the extreme carboxy-terminal residue is crucial. Deletion of this single residue abolished particle assembly almost completely; most substitutions were strongly inhibitory. Site-directed mutations in the carboxy terminus of M were also incorporated into the MHV genome by targeted recombination. The results supported a critical role for this domain of M in viral assembly, although the M carboxy terminus was more tolerant of alteration in the complete virion than in virus-like particles, likely because of the stabilization of virions by additional intermol...Continue Reading

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Citations

May 9, 2000·Journal of Virology·C A de HaanP J Rottier
Mar 29, 2003·Journal of Virology·Bert Jan HaijemaPeter J M Rottier
Aug 3, 2010·Phytotherapy Research : PTR·Huawei LiGuojuan Wu
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Apr 10, 2015·Viruses·Makoto Ujike, Fumihiro Taguchi
Jan 1, 2010·Frontiers of Agriculture in China·Xiaobo ZhangYuan Liu
Aug 28, 2020·Infection and Drug Resistance·Amin Sadeghi DousariNaghmeh Satarzadeh
Oct 29, 1998·The Journal of Biological Chemistry·C A de HaanP J Rottier
Aug 21, 2007·Journal of Medical Virology·Xiaonan FangLinbai Ye
May 15, 2020·Pathogens·Shasha LiHaixue Zheng
Mar 29, 2003·Journal of Virology·Lili Kuo, Paul S Masters
Oct 15, 2003·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Aug 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Boyd YountRalph S Baric
Aug 20, 2010·Journal of Virology·Ariel L ArndtBrenda G Hogue
Feb 16, 2010·The Journal of Biological Chemistry·Ying-Tzu TsengChin-Tien Wang
May 11, 2013·Acta Veterinaria Hungarica·Wei YangXiaofeng Ren
Feb 26, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhiwei YangYujie Fu
Jan 1, 2016·Toxicology and Environmental Health Sciences·Simranjeet Singh SekhonYang-Hoon Kim
Aug 7, 2020·American Journal of Physiology. Endocrinology and Metabolism·Emile LevySchohraya Spahis

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