Coronavirus spike glycoprotein, extended at the carboxy terminus with green fluorescent protein, is assembly competent

Journal of Virology
Berend Jan BoschPeter J M Rottier

Abstract

Due to the limited ultrastructural information about the coronavirion, little is known about the interactions acting at the interface between nucleocapsid and viral envelope. Knowing that subtle mutations in the carboxy-terminal endodomain of the M protein are already lethal, we have now probed the equivalent domain of the spike (S) protein by extending it terminally with a foreign sequence of 27 kDa: the green fluorescent protein (GFP). When expressed individually in murine cells, the S-GFP chimeric protein induced the formation of fluorescent syncytia, indicating that it was synthesized and folded properly, trimerized, and transported to the plasma membrane, where it exhibited the two key S protein functions, i.e., interaction with virus receptor molecules and membrane fusion. Incorporation into virus-like particles demonstrated the assembly competence of the chimeric spike protein. The wild-type S gene of mouse hepatitis coronavirus (MHV) was subsequently replaced by the chimeric construct through targeted recombination. A viable MHV-SGFP was obtained, infection by which could be visualized by the fluorescence induced. The efficiency of incorporation of the chimeric protein into particles was, however, reduced relative to th...Continue Reading

References

Jun 1, 1992·Journal of Virology·J A FosmireS Makino
Jan 1, 1990·Advances in Experimental Medicine and Biology·H VennemaW J Spaan
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O Elroy-Stein, B Moss
Jan 1, 1987·Advances in Experimental Medicine and Biology·K V HolmesM F Frana
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P RottierG Warren
Oct 1, 1995·The Journal of Cell Biology·D J OpsteltenP J Rottier
Dec 1, 1993·Journal of Virology·D J OpsteltenP J Rottier
Jul 11, 1998·Journal of Virology·C A de HaanP J Rottier
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·H GaroffD J Opstelten
Jul 4, 2001·Journal of Biotechnology·L EnjuanesC Sanchez
Jan 5, 2002·Journal of Virology·Kristopher M CurtisRalph S Baric
Oct 15, 2003·Journal of Virology·Cornelis A M de HaanPeter J M Rottier

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Citations

Sep 29, 2005·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Susan R Weiss, Sonia Navas-Martin
Jun 22, 2018·Journal of Virology·Mathieu DubéPierre J Talbot
Sep 6, 2018·PloS One·Antonina NaskalskaKrzysztof Pyrc

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