Coronin 1C harbours a second actin-binding site that confers co-operative binding to F-actin.

The Biochemical Journal
Keefe T ChanJames E Bear

Abstract

Dynamic rearrangement of actin filament networks is critical for cell motility, phagocytosis and endocytosis. Coronins facilitate these processes, in part, by their ability to bind F-actin (filamentous actin). We previously identified a conserved surface-exposed arginine (Arg(30)) in the β-propeller of Coronin 1B required for F-actin binding in vitro and in vivo. However, whether this finding translates to other coronins has not been well defined. Using quantitative actin-binding assays, we show that mutating the equivalent residue abolishes F-actin binding in Coronin 1A, but not Coronin 1C. By mutagenesis and biochemical competition, we have identified a second actin-binding site in the unique region of Coronin 1C. Interestingly, leading-edge localization of Coronin 1C in fibroblasts requires the conserved site in the β-propeller, but not the site in the unique region. Furthermore, in contrast with Coronin 1A and Coronin 1B, Coronin 1C displays highly co-operative binding to actin filaments. In the present study, we highlight a novel mode of coronin regulation, which has implications for how coronins orchestrate cytoskeletal dynamics.

References

May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L E Greene, E Eisenberg
Apr 4, 2003·Biological & Pharmaceutical Bulletin·Teruaki OkuSatoshi Toyoshima
Jul 20, 2005·The Journal of Biological Chemistry·Liang CaiJames E Bear
Jul 30, 2005·Current Biology : CB·Nicole S BryceAlissa M Weaver
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Richard A KammererMichel O Steinmetz
Feb 10, 2006·Cell Research·Chang Zhen LiuSen Fang Sui
Jun 30, 2006·Trends in Cell Biology·Andrea C Uetrecht, James E Bear
Oct 25, 2006·The Journal of Cell Biology·William M BrieherTimothy J Mitchison
Feb 6, 2007·Experimental Cell Research·André RosentreterChristoph S Clemen
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eric A VitriolJames E Bear
Apr 26, 2007·Journal of Cell Science·Liang CaiJames E Bear
Jan 15, 2008·The Journal of Pathology·Dr ThalCs Clemen
Apr 14, 2009·Current Biology : CB·Chikio ChanThomas D Pollard
Dec 25, 2009·The Journal of Biological Chemistry·Kazuya TsujitaTadaomi Takenawa
May 11, 2010·Trends in Biochemical Sciences·Christian U StirnimannChristoph W Müller
Sep 4, 2010·The Journal of Biological Chemistry·Meghal GandhiBruce L Goode
Apr 2, 2011·The Journal of Biological Chemistry·Su-Ling LiuBrad J Nolen

❮ Previous
Next ❯

Citations

Feb 16, 2013·Neuro-oncology·Anja ZiemannChristoph S Clemen
Dec 15, 2015·Cytoskeleton·Rashmi SrivastavaAmogh A Sahasrabuddhe
Dec 15, 2015·Nature Cell Biology·Jasmine V G AbellaMichael Way
Sep 29, 2015·Scientific Reports·Karthic SwaminathanAngelika A Noegel
Aug 9, 2016·American Journal of Human Genetics·Seyyedmohsen HosseinibarkooieBrunhilde Wirth
Jul 15, 2016·PLoS Pathogens·Kartik S BaneFriedrich Frischknecht
May 16, 2018·Journal of Neuroinflammation·Sergey KalininDouglas L Feinstein
Aug 13, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julien SalamunInari Kursula
Dec 8, 2017·Physiological Reviews·Nicolas Molinie, Alexis Gautreau
Feb 27, 2020·International Journal of Molecular Sciences·Tohnyui Ndinyanka FabriceJean Pieters

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence

Software Mentioned

ImageJ
Excel
Graphpad Prism
Prism GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.