Correct assignment of lipophilic dye mixtures? A case study for high-performance thin-layer chromatography-mass spectrometry and performance data for the TLC-MS Interface

Journal of Chromatography. a
Gertrud Morlock, Neil Brett

Abstract

The TLC-MS Interface, the successor of the ChromeXtract, has been available for elution head-based coupling of high-performance thin-layer chromatography with mass spectrometry (HPTLC-MS) since 2009, and is meanwhile widespread in use, mainly for compound confirmation. Until now, quantitative performance data has not been reported in detail and thus were investigated in this study. The performance data of HPTLC-electrospray ionization (ESI)-MS via the TLC-MS Interface showed good mean precisions (%RSD, n=5) for 6 dyes in a commercially available dye mixture investigated at two different concentrations (7.3% for the 1:8 dilution, and 10.1% for the 1:16 diluted) in a selected worst case scenario. The respective mean precisions of absorbance measurements were ≤1.3%. For calibrations by HPTLC-ESI-MS, the mean determination coefficient was 0.9975 for the 6 dyes (versus 0.9997 for absorbance measurement). HPTLC-MS analysis revealed the incorrect assignment of components in two commercially available dye mixtures. Using an additional software (MassWorks) that delivered a 100 times increased mass accuracy, the proposal of molecular formulae was shown to be obtainable under certain conditions with a low resolution single quadrupole mass...Continue Reading

References

Nov 25, 2003·Analytical and Bioanalytical Chemistry·Heinrich Luftmann
Jul 28, 2006·Journal of Chromatography. a·Ute Jautz, Gertrud Morlock
Aug 17, 2006·Analytical and Bioanalytical Chemistry·Alexander Alpmann, Gertrud Morlock
Jan 16, 2007·Journal of Chromatography. a·Gertrud Morlock, Yoshihisa Ueda
Oct 30, 2007·Rapid Communications in Mass Spectrometry : RCM·Heinrich LuftmannGertrud E Morlock
May 25, 2010·Journal of Chromatography. a·Gertrud Morlock, Wolfgang Schwack
Sep 14, 2012·Journal of AOAC International·Joseph Sherma
Jul 30, 2014·Analytical Chemistry·Gertrud E Morlock, Ines Klingelhöfer
Aug 26, 2014·Journal of Chromatography. a·Ines Klingelhöfer, Gertrud E Morlock

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Citations

Oct 17, 2015·Journal of the American Society for Mass Spectrometry·Gregory T WinterWilliam R LaCourse
Mar 9, 2018·Analytical and Bioanalytical Chemistry·Wolfgang SchwackGertrud Morlock
Oct 29, 2019·Rapid Communications in Mass Spectrometry : RCM·Tim T Häbe, Gertrud E Morlock
Feb 18, 2017·Analytical Chemistry·Dimitri Fichou, Gertrud E Morlock
Oct 20, 2021·Journal of Agricultural and Food Chemistry·Dániel KrüzselyiÁgnes M Móricz

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