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Correcting a SHAPE-directed RNA structure by a mutate-map-rescue approach

bioRxiv

Jan 22, 2014

Siqi TianRhiju Das

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Abstract

The three-dimensional conformations of non-coding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure infere...read more

Mentioned in this Paper

RNA, Untranslated
Mapping, Vessel
Landscapes
RNA, Ribosomal, 16S
Oligonucleotide Primers
Validation
Ribosomes
Single Nucleotide Polymorphism
Three-dimensional
Primer Extension
Paper Details
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  • Correcting a SHAPE-directed RNA structure by a mutate-map-rescue approach

    bioRxiv

    Jan 22, 2014

    Siqi TianRhiju Das

    PMID: 990001966

    DOI: 10.1101/001966

    Abstract

    The three-dimensional conformations of non-coding RNAs underpin their biochemical functions but have largely eluded experimental characterization. Here, we report that integrating a classic mutation/rescue strategy with high-throughput chemical mapping enables rapid RNA structure infere...read more

    Mentioned in this Paper

    RNA, Untranslated
    Mapping, Vessel
    Landscapes
    RNA, Ribosomal, 16S
    Oligonucleotide Primers
    Paper Details
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