Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy

Journal of Microscopy
J JanácekIda Erzen

Abstract

Confocal microscopy is a suitable method for measurements and visualization of skeletal muscle fibres and the neighbouring capillaries. When using 3D images of thick sections the tissue deformation effects should be avoided. We studied the deformation in thick sections of the rat skeletal muscle from complete stacks of images captured with confocal microscope. We measured the apparent thickness of the stacks and compared it to the slice thickness deduced from calibrated microtome settings. The ratio of both values yielded the axial scaling factor for every image stack. Careful sample preparation and treatment of the tissue cryosections with cold Ringer solution minimize the tissue deformation. We conclude that rescaling by the inverse of the axial scaling factor of the stack of optical slices in the direction of the microscope optical axis satisfactorily corrects the axial deformation of skeletal muscle samples.

References

Mar 21, 2002·Journal of Microscopy·K A Dorph-PetersenH J Gundersen
Jan 7, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jirí JanácekIda Erzen
Oct 16, 2010·Physiological Research·I ErženL Kubínová
Dec 15, 2010·Microvascular Research·Jiří JanáčekIda Eržen

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Citations

May 16, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Lucie KubínováJiří Janáček
Jun 19, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Erika CvetkoIda Eržen
Oct 4, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Brian GlancyRobert S Balaban
Jan 15, 2014·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Mohamad Feras Marqa, Serge Mordon
Jul 16, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Anna Puigdellívol-SánchezAlberto Prats-Galino
Mar 7, 2015·Cell and Tissue Research·Lucie Kubínová, Jiří Janáček
Nov 3, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ida ErženErika Cvetko
Sep 21, 2017·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Daniel HadrabaKarel Jelen
Sep 2, 2019·Histochemistry and Cell Biology·Nejc UmekIda Eržen
Aug 14, 2019·Journal of Microencapsulation·Mitchell A JohnsonHarald D H Stöver
Mar 19, 2021·Physiological Reviews·Brian Glancy, Robert S Balaban

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