Correction-free remotely scanned two-photon in vivo mouse retinal imaging

Light, Science & Applications
Adi Schejter Bar-NoamShy Shoham

Abstract

Non-invasive fluorescence retinal imaging in small animals is an important requirement for an array of translational vision applications. The in vivo two-photon imaging of the mouse retina may enable the long-term investigation of the structure and function of healthy and diseased retinal tissue. However, to date, this has only been possible using relatively complex adaptive-optics systems. Here, the optical modeling of the murine eye and of the imaging system is used to achieve correction-free two-photon microscopy through the pupil of a mouse eye to yield high-quality, optically sectioned fundus images. By remotely scanning the focus using an electronically tunable lens, high-resolution three-dimensional fluorescein angiograms and cellular-scale images are acquired, thus introducing a correction-free baseline performance level for two-photon in vivo retinal imaging. Moreover, the system enables functional calcium imaging of repeated retinal responses to light stimulation using the genetically encoded indicator, GCaMP6s. These results and the simplicity of the new add-on optics are an important step toward several structural, functional, and multimodal imaging applications that will benefit from the tight optical sectioning an...Continue Reading

References

Jan 1, 1985·Vision Research·S Remtulla, P E Hallett
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·W Denk, P B Detwiler
Jun 13, 2003·Biomedical Engineering Online·Thomas A PologrutoKarel Svoboda
Jan 28, 2004·The Journal of Cell Biology·Yoshikazu ImanishiKrzysztof Palczewski
Apr 13, 2007·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·François C DeloriUNKNOWN American National Standards Institute
May 26, 2007·Investigative Ophthalmology & Visual Science·Michel PaquesJosé-Alain Sahel
May 6, 2002·Optics Express·Austin RoordaMelanie Campbell
Sep 1, 1980·Applied Optics·R H WebbO Pomerantzeff
May 13, 2010·Journal of Biomedical Optics·Emilio J GualdaPablo Artal
Jan 20, 2011·Investigative Ophthalmology & Visual Science·Christopher Kai-shun LeungDennis Shun Chiu Lam
Jan 29, 2011·Journal of Ophthalmology·Emily A GibsonMalik Y Kahook
Feb 25, 2011·Journal of Neurophysiology·Kevin L Briggman, Thomas Euler
Mar 19, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bart G BorghuisLoren L Looger
Apr 13, 2011·Biomedical Optics Express·Ying GengDavid R Williams
May 27, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yanbin V WangJonathan B Demb
Jun 28, 2011·Nature Neuroscience·Elena DreostiLeon Lagnado
Jul 14, 2011·Biomedical Optics Express·Benjamin F GreweFritjof Helmchen
Apr 10, 2012·The Journal of Physical Chemistry. B·Zakhariya ManevitchItzhak Hemo
May 11, 2012·Biomedical Optics Express·Ying GengDavid R Williams
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
Jan 12, 2013·Investigative Ophthalmology & Visual Science·Jelena Marie KezicPaul G McMenamin
Feb 15, 2013·Journal of Neurophysiology·Lu YinWilliam H Merigan
Feb 16, 2013·Biomedical Optics Express·Jonathan J LiuJames G Fujimoto
Feb 28, 2013·Nature Communications·Inna Reutsky-GefenShy Shoham
Mar 28, 2013·Current Eye Research·Matthias KlemmDietrich Schweitzer
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim
Sep 7, 2013·Biomedical Optics Express·Robin SharmaJennifer J Hunter
Jan 1, 2012·Translational Vision Science & Technology·Adi SchejterShy Shoham

❮ Previous
Next ❯

Citations

Dec 6, 2018·Journal of Biomedical Optics·İkbal ŞencanSava Sakadžić
Mar 7, 2019·Light, Science & Applications·Yan-Jie ZhaoDan Zhu
Sep 17, 2019·Annual Review of Vision Science·Jennifer J HunterJesse B Schallek
Mar 12, 2020·Scientific Reports·Zhijian ZhaoThomas Euler
Mar 25, 2016·Light, Science & Applications·Nizan MeitavShy Shoham
Aug 31, 2019·Biomedical Optics Express·Gopal Swamy JayabalanJoseph T Fan
Aug 10, 2018·BioTechniques·Wataru IkedaAkiyoshi Uemura
Nov 16, 2019·Lasers in Medical Science·Pranoy Sahu, Nirmal Mazumder
Dec 5, 2019·Light, Science & Applications·Mengke YangHongbo Jia
Oct 1, 2019·Biomedical Optics Express·Daniel J WahlMarinko V Sarunic
Aug 28, 2021·Micromachines·Younghoon ParkKyungsik Eom

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence imaging

Software Mentioned

2PAOSLO
ScanImage
Adobe Photoshop
Matlab
ZEMAX
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.