Correction of chronic hyperglycemia with vanadate, but not with phlorizin, normalizes in vivo glycogen repletion and in vitro glycogen synthase activity in diabetic skeletal muscle

The Journal of Clinical Investigation
L Rossetti, M R Lauglin

Abstract

Vanadate has insulin-like activity in vitro and in vivo. To characterize the in vivo mechanism of action of vanadate, we examined meal tolerance, insulin-mediated glucose disposal, in vivo liver and muscle glycogen synthesis, and in vitro glycogen synthase activity in 90% partially pancreatectomized rats. Four groups were studied: group I, sham-operated controls; group II, diabetic rats; group III, diabetic rats treated with vanadate; and group IV, diabetic rats treated with phlorizin. Insulin sensitivity, assessed with the euglycemic hyperinsulinemic clamp technique in awake, unstressed rats, was reduced by approximately 28% in diabetic rats. Both vanadate and phlorizin treatment completely normalized meal tolerance and insulin-mediated glucose disposal. Muscle glycogen synthesis was reduced by approximately 80% in diabetic rats (P less than 0.01) and was completely restored to normal by vanadate, but not by phlorizin treatment. Glycogen synthase activity was reduced in skeletal muscle of diabetic rats (P less than 0.05) compared with controls and was increased to supranormal levels by vanadate treatment (P less than 0.01). Phlorizin therapy did not reverse the defect in muscle glycogen synthase. These results suggest that (a)...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·Y Le Marchand-Brustel, P Freychet
Sep 24, 1979·Life Sciences·E L TolmanR Partridge
May 1, 1988·Endocrinology·W C DuckworthD E Peavy
Oct 1, 1987·The Journal of Clinical Investigation·L RossettiR A DeFronzo
May 1, 1986·The Journal of Clinical Endocrinology and Metabolism·S LilliojaC Bogardus
May 1, 1987·The Journal of Clinical Investigation·L RossettiR A DeFronzo
Sep 1, 1985·Metabolism: Clinical and Experimental·S BevilacquaR A DeFronzo
May 1, 1972·Analytical Biochemistry·D P GilboeF Q Nuttall
Jun 1, 1983·The Journal of Clinical Investigation·S Bonner-WeirG C Weir
Apr 1, 1984·The Journal of Clinical Investigation·C BogardusD Mott
Jul 1, 1983·Diabetes·L D KatzR A DeFronzo
Oct 1, 1981·The Journal of Clinical Investigation·O G KoltermanJ M Olefsky

❮ Previous
Next ❯

Citations

Sep 4, 1999·BioFactors·K H Thompson
Jun 1, 1992·Diabetologia·S M BrichardJ C Henquin
Dec 1, 1992·Diabetologia·R P Robertson, D J Klein
Dec 6, 1995·Molecular and Cellular Biochemistry·S K PandeyA K Srivastava
Dec 6, 1995·Molecular and Cellular Biochemistry·J F YaleB I Posner
Aug 1, 1995·Trends in Pharmacological Sciences·S M Brichard, J C Henquin
Feb 27, 1998·Journal of the American College of Nutrition·S VermaJ H McNeill
Jun 25, 1999·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·V BadmaevM Majeed
Dec 21, 2004·Diabetic Medicine : a Journal of the British Diabetic Association·A K Srivastava, M Z Mehdi
Apr 1, 1991·The Journal of Clinical Investigation·J MeyerovitchC R Kahn
Jan 1, 1992·The Journal of Clinical Investigation·A Giaccari, L Rossetti
Jun 1, 1994·The Journal of Clinical Investigation·G BodenL Rossetti
May 1, 1997·The Journal of Clinical Investigation·M HawkinsL Rossetti
Jul 1, 1995·Prostaglandins·A JawerbaumM A de Gimeno
Feb 24, 2012·Journal of Diabetes·Nirav DhaneshaMukul Jain
Jun 25, 1999·Metabolism: Clinical and Experimental·M MatsudaR A DeFronzo
Feb 24, 1998·Metabolism: Clinical and Experimental·P SbracciaG Tamburrano
May 1, 1993·Diabetes Research and Clinical Practice·M C CamJ H McNeill
Apr 1, 1990·Metabolism: Clinical and Experimental·L RossettiR Cordera
Jan 1, 1994·Medical Hypotheses·J E Sprietsma, G E Schuitemaker

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