Correlated alternative side chain conformations in the RNA-recognition motif of heterogeneous nuclear ribonucleoprotein A1

Nucleic Acids Research
Jacqueline VitaliRui-Ming Xu

Abstract

The RNA-recognition motif (RRM) is a common and evolutionarily conserved RNA-binding module. Crystallographic and solution structural studies have shown that RRMs adopt a compact alpha/beta structure, in which four antiparallel beta-strands form the major RNA-binding surface. Conserved aromatic residues in the RRM are located on the surface of the beta-sheet and are important for RNA binding. To further our understanding of the structural basis of RRM-nucleic acid interaction, we carried out a high resolution analysis of UP1, the N-terminal, two-RRM domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), whose structure was previously solved at 1.75-1.9 A resolution. The two RRMs of hnRNP A1 are closely related but have distinct functions in regulating alternative pre-mRNA splice site selection. Our present 1.1 A resolution crystal structure reveals that two conserved solvent-exposed phenylalanines in the first RRM have alternative side chain conformations. These conformations are spatially correlated, as the individual amino acids cannot adopt each of the observed conformations independently. These phenylalanines are critical for nucleic acid binding and the observed alternative side chain conformations may serve as a...Continue Reading

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Citations

Jun 23, 2004·European Journal of Biochemistry·Masayuki NakanishiYukio Kitade
Aug 12, 2009·Molecular and Cellular Biology·Hazeem L Okunola, Adrian R Krainer
Sep 26, 2013·International Journal of Molecular Sciences·Jacques Jean-PhilippeMassimo Caputi
May 13, 2010·Journal of Molecular Biology·Robert A HomTatiana G Kutateladze
Apr 25, 2006·Biochemical and Biophysical Research Communications·Massimo SquatritoGiulio F Draetta
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Apr 24, 2003·Science's STKE : Signal Transduction Knowledge Environment·Paul Lasko
Sep 25, 2017·Nature Communications·Nana Diarra Dit KontéFrédéric H-T Allain
Jan 25, 2020·Current Protein & Peptide Science·Owen M Price, Joan M Hevel

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