PMID: 6982303Nov 1, 1982Paper

Correlated expression of T cell growth factor dependence, sensitivity to Vicia villosa lectin, and cytolytic activity in hybrids between cytolytic T cells and T lymphomas

The Journal of Experimental Medicine
A ConzelmannM Nabholz

Abstract

Somatic cell fusion between cytolytically active, T cell growth factor- (TCGF) dependent murine T cell lines (CTL lines) and noncytolytic, TCGF-independent murine T lymphoma lines has yielded two types of somatic cell hybrids (5): cytolytic hybrids, growth of which is dependent on TCGF, and hybrids with very weak or undetectable cytolytic activity which grow at the same rate with or without TCGF. Here we report that the former can produce stable variants that resemble the latter type. Some of these TCGF-independent variants still have TCGF receptors. High susceptibility to the cytotoxic effects of Vicia villosa lectin, a marker distinguishing the parental CTL lines from T lymphomas, is expressed by the TCGF-dependent hybrids but not by the TCGF-independent variants. The two types of hybrids also differ in the expression of surface glycoproteins. We propose that there exists a genetic element in the CTL line that represses the TCGF-independent replication mechanism of the T lymphoma parent in the TCGF-dependent hybrids and that this genetic element is lost or switched off in the TCGF-independent variants.

References

Aug 1, 1979·European Journal of Immunology·H von BoehmerW Haas
Jul 14, 1977·Nature·S Gillis, K A Smith
Aug 1, 1979·European Journal of Immunology·A CoutinhoJ Andersson
Mar 1, 1976·The Journal of Cell Biology·C G GahmbergL C Andersson
Jan 1, 1979·The Journal of Experimental Medicine·P E BakerK A Smith
Jul 19, 1979·Nature·E L Larsson, A Coutinho
Nov 1, 1979·Somatic Cell Genetics·J DeschatretteM C Weiss
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Nov 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W Taylor, B K Milthorpe
Apr 1, 1980·Cell·J DeschatretteM C Weiss
Nov 1, 1980·The Journal of Experimental Medicine·S ShimizuR T Smith
Oct 2, 1980·Nature·M NabholzJ P Johnson
Nov 1, 1980·European Journal of Immunology·A ConzelmannM Nabholz
Nov 1, 1981·The Journal of Experimental Medicine·J E GootenbergR C Gallo
Nov 1, 1981·The Journal of Experimental Medicine·R J RobbK A Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.