PMID: 9445017Jan 28, 1998Paper

Correlated template-switching events during minus-strand DNA synthesis: a mechanism for high negative interference during retroviral recombination

Journal of Virology
J A AndersonW S Hu

Abstract

Two models for the mechanism of retroviral recombination have been proposed: forced copy choice (minus-strand recombination) and strand displacement-assimilation (plus-strand recombination). Each minus-strand recombination event results in one template switch, whereas each plus-strand recombination event results in two template switches. Recombinant proviruses with one and more than one template switches were previously observed. Recombinants with one template switch were generated by minus-strand recombination, while recombinants containing more than one template switch may have been generated by plus-strand recombination or by correlated minus-strand recombination. We recently observed that retroviral recombination exhibits high negative interference whereby the frequency of recombinants containing multiple template-switching events is higher than expected. To delineate the mechanism that generates recombinants with more than one template switch, we devised a system that permits only minus-strand recombination. Two highly homologous vectors, WH204 and WH221, containing eight different restriction site markers were used. The primer binding site (PBS) of WH221 was deleted; although reverse transcription cannot initiate from WH2...Continue Reading

References

Jul 1, 1979·Journal of Virology·M Linial, S Brown
Jan 1, 1979·Molecular & General Genetics : MGG·R A JorgensenW S Reznikoff
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P KellamB A Larder
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T ShiodaC Cheng-Mayer
Apr 1, 1992·Journal of Virology·P WesterveltL Ratner
Jul 1, 1992·Journal of Virology·W S Hu, H M Temin
Nov 30, 1990·Science·W S Hu, H M Temin
Jan 1, 1990·Annual Review of Genetics·R A Katz, A M Skalka
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W S Hu, H M Temin
Dec 1, 1989·AIDS·J A McKeatingR A Weiss
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H Xu, J D Boeke
Mar 1, 1989·Journal of Virology·F ClavelR Repaske
Jul 1, 1972·Virology·S Kawai, H Hanafusa
Oct 1, 1974·The Journal of General Virology·J L RiggsE H Lennette
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R L White, M S Fox
Jun 1, 1984·Molecular and Cellular Biology·S Kawai, M Nishizawa
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·G A Pulsinelli, H M Temin
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M TisdaleB A Larder
Jan 1, 1994·Journal of Virology·J S JonesH M Temin

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Citations

Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J Kim, C Kao
Jul 4, 2001·The Journal of Biological Chemistry·M BalakrishnanR A Bambara
Oct 9, 2002·The Journal of Biological Chemistry·Ricardo H RodaRobert A Bambara
Feb 14, 2006·Journal of Virology·Christian Lanciault, James J Champoux
Jan 6, 2007·The Journal of Biological Chemistry·Lu GaoRobert A Bambara
Nov 16, 2004·The Journal of Biological Chemistry·Christian Lanciault, James J Champoux
Oct 22, 2002·Journal of Virology·Jianling ZhuangJoseph P Dougherty
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Sep 26, 2014·Viruses·Jonathan M O Rawson, Louis M Mansky
Apr 8, 2000·Journal of Biomedical Science·J G Mikkelsen, F S Pedersen
Jan 11, 2000·Journal of Virology·A E JetztJ P Dougherty
Oct 12, 2016·Viruses·Lizna M AliFarah Mustafa
Oct 12, 2005·Journal of Molecular Biology·Mark Nils HansonRobert A Bambara
Jun 12, 2021·Molecular Biology and Evolution·Claudine Bleykasten-GrosshansJoseph Schacherer

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