Correlating global gene regulation to angiogenesis in the developing chick extra-embryonic vascular system.

PloS One
Sophie JaverzatMartin Hagedorn

Abstract

Formation of blood vessels requires the concerted regulation of an unknown number of genes in a spatial-, time- and dosage-dependent manner. Determining genes, which drive vascular maturation is crucial for the identification of new therapeutic targets against pathological angiogenesis. [corrected] We accessed global gene regulation throughout maturation of the chick chorio-allantoic membrane (CAM), a highly vascularized tissue, using pan genomic microarrays. Seven percent of analyzed genes showed a significant change in expression (>2-fold, FDR<5%) with a peak occurring from E7 to E10, when key morphogenetic and angiogenic genes such as BMP4, SMO, HOXA3, EPAS1 and FGFR2 were upregulated, reflecting the state of an activated endothelium. At later stages, a general decrease in gene expression occurs, including genes encoding mitotic factors or angiogenic mediators such as CYR61, EPAS1, MDK and MYC. We identified putative human orthologs for 77% of significantly regulated genes and determined endothelial cell enrichment for 20% of the orthologs in silico. Vascular expression of several genes including ENC1, FSTL1, JAM2, LDB2, LIMS1, PARVB, PDE3A, PRCP, PTRF and ST6GAL1 was demonstrated by in situ hybridization. Up to 9% of the CA...Continue Reading

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Citations

Aug 21, 2014·Angiogenesis·Patrycja Nowak-SliwinskaM Luisa Iruela-Arispe
Oct 18, 2014·Journal of Proteome Research·Andrei TurtoiVincent Castronovo
Mar 6, 2010·BMC Research Notes·Maurice BalkeMartin Hagedorn
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Aug 24, 2013·Blood·Martin Hagedorn
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Jul 13, 2017·Expert Opinion on Therapeutic Patents·Thomas H Graham
May 17, 2018·Angiogenesis·Patrycja Nowak-SliwinskaArjan W Griffioen
Mar 24, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Bettina PitterEloi Montanez
Oct 19, 2012·Angiogenesis·Patricia FergelotAndreas Bikfalvi
Dec 12, 2013·Oncotarget·Prisca ExertierMartin Hagedorn

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Datasets Mentioned

BETA
GSE11636
GSM294993

Methods Mentioned

BETA
SMO
PCR
transfection
light microscopy

Software Mentioned

Excel
Elements AR
Affymetrix
BLAST
GCOS
NIS
DAVID
Java
DAVID ( Database for Annotation , Visualization and Integrated...
TreeView

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