Correlation analysis of intracellular and secreted cytokines via the generalized integrated mean fluorescence intensity.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Parisa ShooshtariRyan R Brinkman

Abstract

The immune response in humans is usually assessed using immunogenicity assays to provide biomarkers as correlates of protection (CoP). Flow cytometry is the assay of choice to measure intracellular cytokine staining (ICS) of cell-mediated immune (CMI) biomarkers. For CMI analysis, the integrated mean fluorescence intensity (iMFI) was introduced as a metric to represent the total functional CMI response as a CoP. iMFI is computed by multiplying the relative frequency (percent positive) of cells expressing a particular cytokine with the MFI of that population, and correlates better with protection in challenge models than either the percentage or the MFI of the cytokine-positive population. While determination of the iMFI as a CoP can readily be accomplished in animal models that allow challenge/protection experiments, this is not feasible in humans for ethical reasons. As a first step toward extending the iMFI concept to humans, we investigated the correlation of the iMFI derived from a human innate immune response ICS assay with functional cytokine release into the culture supernatant, as innate cytokines need to be released to have a functional impact. Next, we developed a quantitatively more correlative mathematical approach ...Continue Reading

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Citations

Dec 18, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Yingjuan LuChristopher P Leamon
Dec 8, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Anush ArakelyanLeonid Margolis
May 2, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Albert D DonnenbergVera S Donnenberg
Jan 8, 2011·Vaccine·Kristin ScheibleDavid J Topham
Dec 17, 2014·PloS One·Keven HuangAdeela Kamal
Oct 23, 2016·Free Radical Biology & Medicine·Thiruvaimozhi AbimannanSatish Devadas
Jul 3, 2018·Redox Report : Communications in Free Radical Research·Ibraheem HusainAbul Kalam Najmi
Sep 22, 2018·Cytometry. Part B, Clinical Cytometry·John D M Campbell, Alasdair R Fraser
Feb 3, 2018·Journal of Neuroinflammation·Anne M NixonJames R Connor
Dec 17, 2019·Immunological Reviews·Katherine R DobbsArlene E Dent
Jan 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·John Nicholas MelvanPing Zhang
Mar 25, 2018·The Journal of Experimental Medicine·Vivek DuraiKenneth M Murphy
Jun 10, 2015·Nature Immunology·Gary E Grajales-ReyesKenneth M Murphy
Feb 11, 2020·Frontiers in Immunology·Jessica VazquezAleksandar K Stanic
Aug 14, 2019·Nature Immunology·Prachi BagadiaKenneth M Murphy
Aug 8, 2021·International Journal of Molecular Sciences·Xin ZhangVirginia Byers Kraus
Apr 4, 2014·ACS Synthetic Biology·Michael Guinn, Leonidas Bleris
Oct 26, 2021·Frontiers in Immunology·Xin ZhangVirginia B Kraus
Nov 18, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Amy GrahamKewal Asosingh

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