Correlation between 2H NMR side-chain order parameters and sequence conservation in globular proteins

Journal of the American Chemical Society
A MittermaierL E Kay

Abstract

Side-chain 2H NMR relaxation data have been collected for the SH3 domain from the Fyn tyrosine kinase and analyzed with respect to sequence preference and per-residue solvent accessibility. Residues that are highly preferred at a given position show a tendency to be less mobile than average with a coefficient of correlation that is greater than that obtained when side-chain flexibility and solvent accessibility are compared. The same trend is observed for five of six additional proteins considered. This provides evidence for the existence of conserved structural features other than hydrophobic burial that govern side-chain motions. Through examination of an SH3 domain structural alignment, we identify side-chain hydrogen bonding of threonine residues and a specific secondary structural element as potential determinants of protein internal dynamics.

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Citations

Aug 25, 2010·Journal of Biomolecular NMR·Glenn L ButterfossRobert E London
Sep 25, 2012·Journal of Biomolecular NMR·Benjamin StauchTeresa Carlomagno
Apr 21, 2009·Journal of the American Chemical Society·Anthony B LawAndrew L Lee
Aug 4, 2009·Bioinformatics·Pablo Carbonell, Antonio del Sol
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert B BestMichele Vendruscolo
Mar 20, 2012·Molecular Biology and Evolution·Ying Liu, Ivet Bahar
Jun 8, 2013·Evolutionary Applications·Zeynep Nevin GerekSefika Banu Ozkan
Jun 13, 2008·Journal of Molecular Biology·Gregory D FriedlandTanja Kortemme
Nov 26, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Joseph A Marsh, Sarah A Teichmann
Dec 4, 2008·Journal of the American Chemical Society·Vipin AgarwalNikolai R Skrynnikov
Aug 10, 2006·Chemical Reviews·David D BoehrPeter E Wright
Jun 24, 2004·Journal of the American Chemical Society·Robert B BestMartin Karplus
Aug 21, 2010·Journal of the American Chemical Society·Tsutomu YamaneMitsunori Ikeguchi
May 23, 2014·Journal of the American Chemical Society·Rajitha Rajeshwar TMarimuthu Krishnan
Sep 17, 2013·Journal of the American Chemical Society·Mark V Berjanskii, David S Wishart

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