Correlation between catecholamine secretion from bovine isolated chromaffin cells and [3H]-ouabain binding to plasma membranes

British Journal of Pharmacology
D Aunis, A G García

Abstract

1 Secretion of catecholamines (CA) evoked by ouabain, chlormadinone acetate (CMA), phenoxybenzamine (Pbz) and vanadate, four agents known to inhibit Na(+), K(+)-dependent Mg(2+)-activated adenosine triphosphatase (ATPase) activity has been studied in suspensions of bovine isolated adrenal medullary cells.2 Acetylcholine (ACh) evoked a 5 fold increase of the basal CA secretion from isolated cells suspended in oxygenated Krebs-bicarbonate solution kept at 27 degrees C. Secretion was antagonized by Ca(2+)-deprivation or hexamethonium, indicating good functional viability of the cells.3 Ouabain (10(-7) to 10(-4) M) evoked a progressive, dose-dependent release of CA from cell suspensions. Study of the time course of the secretory response for 2 h allowed the separation of two components in the secretory response at all doses studied: a slow initial component (0.011 pg/min CA) and a second faster component (0.032 pg/min CA).4 CMA evoked a clear-cut CA secretory response. The ED(50) for CMA was 10(-4) M, as compared to 3 x 10(-6) M for ouabain. Pbz and vanadate did not induce CA release.5 [(3)H]-ouabain was taken up and bound to intact isolated cells by a non-saturable binding process. However, in semi-purified plasma membranes from b...Continue Reading

References

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Citations

Jun 13, 1996·European Journal of Pharmacology·M T De la FuenteA G García
Jan 1, 1983·Journal of the Autonomic Nervous System·B G LivettM H Lindenbaum
Jan 1, 1989·Progress in Neurobiology·T L Török
Jun 1, 1994·Journal of Autonomic Pharmacology·A L Cahill, R L Perlman
Oct 1, 1990·Cell Calcium·P S Liu, L S Kao
Nov 1, 1981·British Journal of Pharmacology·A G GarciaP Sanchez-Garcia
Aug 1, 1986·British Journal of Pharmacology·C R Artalejo, A G Garcia
Apr 1, 1984·British Journal of Pharmacology·G AlmazanP Sánchez-García
Jan 3, 2012·Cell Calcium·Antonio G GarcíaJavier García-Sancho
Aug 26, 2016·Pflügers Archiv : European journal of physiology·Ricardo De PascualAntonio G García
Jun 1, 1983·Journal of Autonomic Pharmacology·D A Powis
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Jul 1, 1987·British Journal of Pharmacology·F J AbajoP Sánchez-García

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