PMID: 9525255Apr 3, 1998Paper

Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines

International Journal of Radiation Biology
E DikomeyB Kaina

Abstract

To test the relationship between cell killing and non-repaired DNA strand breaks both in repair proficient and deficient cell lines. Five of the cell lines used are repair competent (CHO, CHO K1, rat rhabdomyosarcoma R1H, mouse balb and normal human fibroblasts), while four display a reduced repair capacity (scid, xrs1, xrs5, AT). Cell survival was determined by colony formation assay. The total number of strand breaks was measured by the alkaline unwinding technique and the numbers of double-strand breaks by constant-field gel electrophoresis. The nine cell lines showed a broad spectrum in radiosensitivity with SF2 values ranging from 0.018 to 0.58. The cell lines did not vary in the number of induced strand breaks, neither for all strand breaks nor for double-strand breaks alone. In contrast, there was a large variation in the number of non-repaired strand breaks measured 24 h after irradiation. Comparison of cell killing with the number of non-repaired breaks measured after a dose of 90 Gy showed no correlation for single-strand breaks (r2=0.29) but a fairly good correlation for double-strand breaks (r2=0.87). This correlation was found to hold both for repair proficient and deficient cell lines. The results obtained strongl...Continue Reading

Citations

Jan 29, 2013·BMC Urology·Diego A PalaciosCharles J Rosser
Feb 28, 2014·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Makoto IharaTakeo Ohnishi
Mar 13, 2009·International Journal of Radiation Biology·Hyewon YounHeon Joo Park
Mar 6, 2010·International Journal of Radiation Biology·Marie-Anne Hervé du PenhoatMark A Hill
Oct 16, 2012·Nuclear Medicine and Biology·Amanda J WeeksDaniel R Lloyd
Apr 15, 2008·International Journal of Radiation Oncology, Biology, Physics·Kerstin BorgmannEkkehard Dikomey
May 15, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Kerstin BorgmannEkkehard Dikomey
Apr 2, 2011·Analytical Biochemistry·Venkatesh H GouthamSadashiva Rao Bola Satish
Nov 26, 2015·Mutation Research. Reviews in Mutation Research·Mohammad Sabbir SiddiquiWayne R Leifert
Dec 11, 2003·The Journal of Veterinary Medical Science·Seiichi WadaNobuhiko Ito
May 9, 2003·The Journal of Veterinary Medical Science·Seiichi WadaNobuhiko Ito
Feb 28, 2008·Radiation Research·I Szumiel
Jan 28, 2011·Radiation Research·Honghong ChenJunran Zhang
Jun 19, 2013·Radiation Research·Yoshihiro FujiiTakamitsu A Kato
Jun 15, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Laura MyllynenMalte Kriegs
Aug 21, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Simon LabanMalte Kriegs
Feb 7, 2012·Journal of Molecular Biology·Kishore K ChiruvellaSathees C Raghavan
Jan 16, 2007·Biochemical and Biophysical Research Communications·Fu-Xiang ZhouYun-Feng Zhou
May 14, 2016·Journal of Radiation Research·Janapriya Saha, Anthony J Davis
Jun 28, 2016·Life Sciences in Space Research·Deepa M SridharanJanice M Pluth
Jul 17, 2016·Journal of Radiation Research·Lei ZhaoYeqing Sun
Mar 31, 2004·Canadian Journal of Physiology and Pharmacology·Didier BoucherDietrich Averbeck
Aug 9, 2017·International Journal of Molecular Sciences·Margund BachMichael Hausmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.