PMID: 8955558Nov 1, 1996Paper

Correlation between cytosolic Ca2+ concentration, protein phosphorylation and platelet secretion

Cell Calcium
L Dalla ViaR Deana

Abstract

Addition of the calcium-ionophore ionomycin to acetylsalicylate-treated platelets suspended in a low Ca2+ concentration-containing medium (about 0.1 microM), induced a dose-dependent (range 0.25-3 microM) and transient increase in the cytosolic Ca2+ concentration ([Ca2+]c). Less than 10% of the maximal releasable amount of serotonin was secreted at [Ca2+]c lower than 1 microM, whereas secretion was almost maximal at [Ca2+]c higher than 2 microM. In all cases the secretion stopped after about 1 min even if the [Ca2+]c was kept constant by repeated small additions of CaCl2 (25-40 microM). A rapid phosphorylation of pleckstrin (47 kDa) and myosin light chain (20 kDa) was found in all cases, whereas a weak phosphorylation of a 27 kDa protein occurred at [Ca2+]c lower than 1.5 microM. Addition of 0.2 mM CaCl2 to platelets pretreated for 4 min with 0.5-1 microM ionomycin brought about a serotonin secretion remarkably lower than obtained by the simultaneous addition of CaCl2 and ionophore. Platelets suspended in a low calcium-containing medium and exposed to ionomycin showed a major increase in tyrosine phosphorylation of 60 and 72 kDa proteins and a slight increment in tyrosine phosphorylation of 115 and 130 kDa proteins. Subsequent ...Continue Reading

References

Sep 24, 1991·Biochimica Et Biophysica Acta·M G DoniA Alexandre
Oct 1, 1991·Current Opinion in Cell Biology·S J Shattil, J S Brugge
Jan 1, 1990·Annual Review of Physiology·T J Rink, S O Sage
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Nov 1, 1994·Trends in Biochemical Sciences·E A ClarkJ S Brugge

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Citations

Jun 3, 1998·Chemico-biological Interactions·L Dalla ViaR Deana

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