PMID: 9436621Jan 22, 1998Paper

Correlation between direct ESR spectroscopic measurements and electromechanical and biochemical assessments of exogenous free radical injury in isolated rat cardiac myocytes

Free Radical Biology & Medicine
M CourtoisL Rochette

Abstract

Reactive free radical species appear to be involved in the ischemic injury of cardiac muscle, although the mechanisms by which oxygen-derived free radicals affect the heart cell function are not known. In the present study, cultured ventricular myocytes were exposed to an exogenous oxygen radical generating system. The myocyte-enriched, primary cultures were prepared from ventricles of new-born rat heart and exposed to a xanthine/xanthine oxidase (X+XO) system. The transmembrane potentials were recorded with glass microelectrodes. Cell contractions were monitored photometrically. The release of lactate dehydrogenase (LDH) in the medium was analysed. Quantitative measurement and the time course of the radical generation were performed by the electron paramagnetic resonance (EPR) spin trapping technique with the spin trap 5,5-dimethyl-1-pyroline-N-oxide (DMPO). We verified that X and XO alone had no significant functional and biochemical effects. The X+XO system produced a rapid decrease in the action potential amplitude. This effect was accompanied by a strong decrease in contractility and spontaneous rate. The time course of these functional defects were correlated with a progressive efflux of LDH from the cardiomyocytes. Prolo...Continue Reading

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Citations

Nov 7, 2000·Free Radical Biology & Medicine·I DurotP Athias
Nov 13, 2004·The Journal of Nutritional Biochemistry·W R LeifertE J McMurchie
Jul 19, 2000·Thorax·L M Heunks, P N Dekhuijzen
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May 19, 2004·British Journal of Pharmacology·Masahiro YamawakiIchiro Hisatome
Mar 17, 2001·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·J MartinF Beyersdorf

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