Correlation between DNA methylation and chronological age of Moso bamboo (Phyllostachys heterocycla var. pubescens)

Botanical Studies
Jin-Ling YuanXiao-Ping Gu

Abstract

Chronological age is the primary consideration when studying the physiological development, aging, and flowering of bamboo. However, it's difficult to determine bamboo's chronological age if the time of germination is unknown. To investigate the chronological age of bamboo from the genomic DNA methylation profile, methylation-sensitive amplification polymorphism (MSAP) was employed to analyze the genomic DNA methylation of Moso bamboo (Phyllostachys heterocycla var. pubescens) from stands of nine germination-ages, using six primer pairs which have previously been shown to yield methylation rates that reflect the age of Moso bamboo. The results showed that the total genomic DNA methylation rates in Moso bamboo at different chronological ages were significantly different, and the increase in genomic DNA methylation rate was consistent with the increase of chronological age. Six primer pairs displayed different genomic DNA methylation rates in Moso bamboo of nine age's group; however, a significantly positive correlation existed among these primer pairs. An integrated index was obtained by performing principal component analysis on the six primer pairs to represent the genomic DNA methylation levels in Moso bamboo of various chron...Continue Reading

Citations

Sep 27, 2018·Plant Biology·S Varga, C D Soulsbury
Aug 12, 2020·Plant Cell Reports·Zijie ZhangYun Li
Feb 3, 2020·Planta·Kanagesswari MuniandySadequr Rahman
Jul 3, 2015·Annals of Botany·Marcin MichalakPaweł Chmielarz
Mar 13, 2014·Molecular Ecology Resources·Andrea M PolanowskiSimon N Jarman

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

SPSS
SPSS16
MSAP

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