PMID: 9532772Apr 9, 1998Paper

Correlation between dynamics, structure and spectral properties of human alpha 1-acid glycoprotein (orosomucoid): a fluorescence approach

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
J R Albani

Abstract

Dynamics of proteins and membranes are usually investigated by red-edge excitation spectra and fluorescence anisotropy. In a viscous or rigid medium, the fluorescence maximum position changes with the excitation wavelength upon red-edge excitation. In addition to the shift in the emission maximum on red edge excitation, fluorescence anisotropy is also known to be dependent on the excitation and emission wavelengths in viscous media. However, this dependence has always been explained by the fact that the fluorophore is rigid, i.e. it does not display any residual motions. The aim of the present work was to check the validity of this latest assumption and to explain the possible origin of the dependence of the anisotropy on both the excitation and emission wavelengths. Therefore, we compared the results obtained from the fluorescence of the Trp residues of two alpha 1-acid glycoproteins (orosomucoid). One protein was purified by chromatographic methods (orosomucoid(c)) and the other was obtained with ammonium sulfate precipitation (orosomucoid(s)). Trp residues of orosomucoidc display free motions while those of orosomucoids are rigid. The general qualitative feature of the excitation anisotropy spectra recorded on both types of ...Continue Reading

References

May 15, 1992·European Journal of Biochemistry·Z WasylewskiK Slizowska
Jan 1, 1991·Methods of Biochemical Analysis·M R Eftink
Oct 1, 1973·Photochemistry and Photobiology·E A BursteinM N Ivkova
May 1, 1967·Analytical Biochemistry·R F Chen
Jun 19, 1984·Biochemistry·J R Lakowicz, S Keating-Nakamoto
Apr 12, 1983·Biochemistry·J R LakowiczA Balter
Apr 30, 1981·Biochemical and Biophysical Research Communications·J R Lakowicz, H Cherek
Jul 1, 1981·Biophysical Journal·E D Matayoshi, A M Kleinfeld
Jan 5, 1995·Biochimica Et Biophysica Acta·M KodícekV Karpenko
Dec 13, 1993·FEBS Letters·A Chattopadhyay, R Rukmini
Feb 1, 1993·The Biochemical Journal·S UrienJ P Tillement
Apr 13, 1993·Biochemistry·A Chattopadhyay, S Mukherjee
Oct 15, 1996·Biochemistry·S GuhaB Bhattacharyya
Dec 6, 1996·Biochimica Et Biophysica Acta·J R Albani
Aug 29, 1997·Biochimica Et Biophysica Acta·J R Albani
Apr 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G Weber, M Shinitzky

❮ Previous
Next ❯

Citations

Jan 30, 2002·Luminescence : the Journal of Biological and Chemical Luminescence·Alexander P Demchenko
Jan 9, 2007·Journal of Photochemistry and Photobiology. B, Biology·Feng ZhangPing Li
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.