Correlation between electric field pulse induced long-lived permeabilization and fusogenicity in cell membranes

Biophysical Journal
J Teissie, C Ramos

Abstract

Electric field pulses have been reported to induce long-lived permeabilization and fusogenicity on cell membranes. The two membrane property alterations are under the control of the field strength, the pulse duration, and the number of pulses. Experiments on mammalian cells pulsed by square wave form pulses and then brought into contact randomly through centrifugation revealed an even stronger analogy between the two processes. Permeabilization was known to affect well-defined regions of the cell surface. Fusion can be obtained only when permeabilized surfaces on the two partners were brought into contact. Permeabilization was under the control of the pulse duration and of the number of pulses. A similar relationship was observed as far as fusion is concerned. But a critical level of local permeabilization must be present for fusion to take place when contacts are created. The same conclusions are obtained from previous experiments on ghosts subjected to exponentially decaying field pulses and then brought into contact by dielectrophoresis. These observations are in agreement with a model of membrane fusion in which the merging of local random defects occurs when the two membranes are brought into contact. The local defects are...Continue Reading

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Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·Mateja GabrijelRobert Zorec
Jun 6, 2000·Advanced Drug Delivery Reviews·J TeissiéM P Rols
May 6, 1999·Bioelectrochemistry and Bioenergetics·M C VernhesJ Teissie
Jun 24, 1999·Bioelectrochemistry and Bioenergetics·S Biswas, S K Guha
Jul 21, 2010·Journal of Visualized Experiments : JoVE·Katja TronteljDamijan Miklavcic
Mar 7, 2002·Analytical Biochemistry·Corinne RamosJustin Teissie
Jul 12, 2014·Bioelectrochemistry·Feriel Siham HamdiBruno Le Pioufle
Jul 11, 2014·Expert Opinion on Drug Delivery·Maša Kandušer, Marko Ušaj
Nov 30, 2013·Scientific Reports·Lea RemsGorazd Pucihar
Mar 23, 2015·The Journal of Membrane Biology·P HenriJustin Teissié
Jan 26, 2006·IEEE Transactions on Nanobioscience·Yinghua SunMartin A Gundersen
Nov 19, 2003·IEEE Transactions on Bio-medical Engineering·Davorka SelDamijan Miklavcic
Apr 12, 2015·Biophysical Journal·Lars H WegnerAude Silve
Mar 29, 2005·Biophysical Journal·Mojca PavlinDamijan Miklavcic
May 9, 2002·FEBS Letters·Corinne RamosJustin Teissié
Oct 29, 2005·Scandinavian Journal of Immunology·F HilpertJ Pfisterer
Mar 19, 2020·International Journal of Molecular Sciences·Yariswamy ManjunathJussuf T Kaifi
Mar 1, 2017·The Journal of Membrane Biology·Claudia MuratoriOlga N Pakhomova
Mar 1, 2013·Journal of Cell Science·Günther GerischEberhard Neumann

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