Correlation between FAK and EGF-Induced EMT in Colorectal Cancer Cells

Journal of Oncology
Kun HuangXuemei Wang

Abstract

Epithelial-mesenchymal transition (EMT) plays an important role in the invasion and metastasis of colorectal cancer, which is mediated by FAK and EGF. However, whether FAK participates in EMT in colorectal cancer cells through the EGF/EGFR signaling pathway remains unknown. The aim of this study was to investigate the effector mechanisms of FAK in the process of EGF-induced EMT in colorectal cancer cells and to determine whether miR-217 is involved in this process. Caco-2 cancer cells were routinely cultured with and without treatment with 100 ng/mL EGF, and changes in cell morphology were observed using an inverted microscope. In addition, a transwell assay was used to detect cell migration under the condition of EGF treatment. The expression of FAK, pFAK, E-cadherin, vimentin, and β actin was assessed by western blotting, and the expression of miR-217 was assessed using real-time PCR. We found that EGF induced EMT in colorectal cancer cells and enhanced cell migration and invasion ability. Moreover, FAK was involved in the EGF-induced EMT of colorectal cancer cells. EGF upregulated the expression of E-cadherin in colorectal cancer cells by activating FAK, and miR-217 was found to participate in EGF-induced EMT in colorectal c...Continue Reading

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Citations

Dec 5, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiManoj Garg
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamed R AbdelaalHesham Haffez

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Methods Mentioned

BETA
electrophoresis
Assay
PCR

Software Mentioned

SPSS

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