Correlation between heat shock proteins, adiponectin, and T lymphocyte cytokine expression in type 2 diabetics

Cell Stress & Chaperones
Fadia F MahmoudFawzia Al-Najjar

Abstract

Type 2 diabetes mellitus (T2DM) features insulin resistance, hyperglycemia, dyslipidemia, overproduction of inflammatory cytokines, and systemic oxidative stress. Here, heat shock proteins Hsp70 and Hsp 90, adiponectin, and heme oxygenase-1 (HO-1, Hsp32) are profiled in peripheral blood mononuclear cells (PBMC) and serum from 25 T2DM patients and 25 healthy control subjects. Cells cultured with phorbol 12-myristate 13-acetate/ionomycin were evaluated by three-color flow cytometry for immunophenotypic biomarkers. Plasma HO-1, Hsp, and adiponectin levels were assayed by enzyme-linked immunosorbent assay (ELISA). Relative to healthy controls, T2DM patients exhibited significantly elevated plasma Hsp70, and representation of T helper immunophenotypes activated to express inflammatory cytokines, including CD4+ IFN-γ+, CD4+ TNF-α+, CD4+ IL-6+, CD4+ IL-1β+ T cells, significantly lower representation of CD4+ IL-10+ T cells, plasma adiponectin and cell-associated HO-1 expression-with no significant differences in plasma Hsp90 between T2DM and healthy controls. Plasma HO-1 and adiponectin in T2DM patients inversely correlated with TNF-α and showed inverse correlation between serum LDL and plasma HO-1. Moreover, TNF-α and Hsp90 in T2DM pa...Continue Reading

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Citations

Oct 20, 2018·International Journal of Molecular Sciences·Katarzyna E Skórzyńska-DziduszkoBożena Leszczyńska-Gorzelak
Apr 22, 2021·BMJ Open Diabetes Research & Care·Alexander S AtkinAlexandra E Butler
Aug 25, 2021·Scientific Reports·Alexander S AtkinAlexandra E Butler

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
enzyme-linked immunosorbent assay
ELISA
FACS

Software Mentioned

CXP cytometer
SPSS

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