Correlation between hepatitis B virus protein and microRNA processor Drosha in cells expressing HBV

Antiviral Research
Min RenHua Tang

Abstract

Drosha regulates the biogenesis of microRNAs (miRNAs) and plays an essential role in the regulation of gene expression. Infection with hepatitis B virus (HBV) causes chronic hepatitis and liver cirrhosis. It is also a major risk factor for hepatocellular carcinoma. Emerging evidence suggests that HBV alters miRNA expression profiles, but the mechanisms underlying this process have not yet been fully elucidated. We therefore examined how HBV affected the production of miRNAs. We found that Drosha mRNA and protein expression were downregulated in cells expressing the HBV genome. This was associated with a reduction in the activity of the Drosha gene promoter. Gene silencing of HBx by RNA interference significantly restored the expression of Drosha. In conclusion, our data show that HBV could downregulate Drosha expression by inhibiting promoter activity, and the transcription factors SP1 and AP-2α may be involved in this process. This provides a new understanding of the mechanism of HBV-induced miRNAs dysregulation.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Dec 19, 2003·Genes & Development·Rui YiBryan R Cullen
Mar 12, 2005·The Journal of Biological Chemistry·Cha-Kyung YounHo Jin You
Jun 10, 2005·Nature·Jun LuTodd R Golub
Sep 6, 2005·Science·Catherine L JoplingPeter Sarnow
Aug 3, 2006·Genes & Development·J Michael ThomsonScott M Hammond
Aug 17, 2006·Nature Reviews. Microbiology·Peter SarnowKaren A Wehner
Dec 26, 2006·Cancer Letters·Mark A Feitelson, Jungmin Lee
Mar 22, 2007·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S MaorH Werner
Apr 3, 2007·Nature Genetics·Madhu S KumarTyler Jacks
May 18, 2007·Annual Review of Genomics and Human Genetics·Tsung-Cheng Chang, Joshua T Mendell
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Jun 11, 2008·Cell Host & Microbe·Eva Gottwein, Bryan R Cullen
Mar 19, 2009·Biochemical and Biophysical Research Communications·Toru Suzuki, Hiroshi Tamada
Jun 23, 2009·Biochemical and Biophysical Research Communications·Jianhui QuMinghua Zhu
May 19, 2010·Annual Review of Microbiology·Rebecca L Skalsky, Bryan R Cullen
Jun 12, 2010·Gastroentérologie Clinique Et Biologique·S Pfeffer, T F Baumert
Jun 23, 2011·Journal of Viral Hepatitis·F JiP Tien

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Citations

Aug 16, 2013·World Journal of Gastroenterology : WJG·Ying-Feng WeiHong-Yan Diao
Apr 16, 2014·The International Journal of Biochemistry & Cell Biology·Megan L FinchBernard A Callus
Oct 30, 2014·Nature Communications·Rajesha RupaimooleAnil K Sood
Mar 2, 2016·International Journal of Molecular Sciences·C Nelson Hayes, Kazuaki Chayama
Oct 14, 2014·World Journal of Gastroenterology : WJG·Hong-Xia Fan, Hua Tang
Jan 13, 2016·World Journal of Gastroenterology : WJG·Shan-Ying WuHsiao-Sheng Liu
Apr 2, 2020·World Journal of Gastroenterology : WJG·Diana Gabriela IacobSimona Maria Ruta
Jan 1, 2014·Molecular & Cellular Oncology·Xiaojie Xu, Qinong Ye
Aug 8, 2021·International Journal of Molecular Sciences·Valeria PietropaoloUgo Moens

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