Correlation between inhibition of myelin basic protein (arginine) methyltransferase by sinefungin and lack of compact myelin formation in cultures of cerebral cells from embryonic mice

Journal of Neuroscience Research
S G AmurR A Pieringer

Abstract

Sinefungin, a known inhibitor of protein methylation, inhibited the myelin basic protein (arginine) methyltransferase activity in homogenates of cultured cerebral cells from embryonic mice. Fifty percent inhibition was achieved with 25 microM sinefungin. Electron microscopic examination of the myelin fraction, isolated by gradient density centrifugation and obtained from untreated cells, revealed numerous ringlike multilamellar membranous substructures that had a major dense line periodicity, compactness, and the general appearance expected of myelin obtained by the same technique from whole brain. Cells treated with 30 microM sinefungin, which inhibits myelin basic protein methyltransferase in broken cell preparations about 60%, produced ringlike structures that were devoid of multilamellar periodicity and compactness reminiscent of the vacuolated myelin observed in subacute combined degeneration and in nitrous-oxide- or cycloleucine-treated animals in which methyltransferase activity is also inhibited. The sinefungin-induced change in multilamellar periodicity cannot be attributed to a lack of myelin basic protein, since the ratio of myelin basic protein to total protein did not decrease in sinefungin-treated cells. This prim...Continue Reading

References

Aug 13, 1979·Biochemical and Biophysical Research Communications·R T BorchardtC O Rutledge
Mar 1, 1976·Journal of Neurochemistry·E Miyamoto
Jun 8, 1973·Biochemical and Biophysical Research Communications·N Sundarraj, S E Pfeiffer
Dec 1, 1974·Journal of Neurochemistry·G M Jones, P R Carnegie
Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Jun 1, 1966·Journal of Ultrastructure Research·U Karlsson
Oct 1, 1980·Biochemical Society Transactions·A J Crang, W Jacobson
Jan 1, 1983·Neurochemistry International·N P GroomeJ Appleby

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Citations

Jan 1, 1993·Journal of Inherited Metabolic Disease·R Surtees
Jan 1, 1988·Molecular Neurobiology·A T Campagnoni, W B Macklin
May 1, 1997·The International Journal of Biochemistry & Cell Biology·S KimW K Paik
Apr 1, 1993·Journal of Neurochemistry·K NakajimaK Mikoshiba
Jul 1, 1988·Journal of Neurochemistry·A T Campagnoni
Aug 20, 2005·Progress in Neuro-psychopharmacology & Biological Psychiatry·Teodoro Bottiglieri
Oct 30, 2007·Birth Defects Research. Part C, Embryo Today : Reviews·Anna E Beaudin, Patrick J Stover
Feb 14, 2008·Medicinal Research Reviews·Yujun George ZhengMasha Goodman
May 24, 1996·The Journal of Biological Chemistry·J D GaryS Clarke
Jul 11, 2012·Medicinal Research Reviews·Keqin Kathy LiY George Zheng
Jun 30, 1987·Biochemical and Biophysical Research Communications·P R YoungC M Waickus
Sep 29, 2006·Pharmacology & Therapeutics·Christopher D KrauseSidney Pestka
Oct 5, 2010·Biochimica Et Biophysica Acta·B Ruthrotha SelviTapas K Kundu

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