Correlation between lifetime heterogeneity and kinetics heterogeneity during chlorophyll fluorescence induction in leaves: 2. Multi-frequency phase and modulation analysis evidences a loosely connected PSII pigment-protein complex

Biochimica Et Biophysica Acta
Nicolae Moise, Ismaël Moya

Abstract

We report the first direct decomposition of the fluorescence lifetime heterogeneity during multiphasic fluorescence induction in dark-adapted leaves by multi-frequency phase and modulation fluorometry (PMF). A very fast component, assigned to photosystem I (PSI), was found to be constant in lifetime and yield, whereas the two slow components, which are strongly affected by the closure of the reaction centers by light, were assigned to PSII. Based on a modified "reversible radical pair" kinetic model with three compartments, we showed that a loosely connected pigment complex, which is assumed to be the CP47 complex, plays a specific role with respect to the structure and function of the PSII: (i) it explains the heterogeneity of PSII fluorescence lifetime as a compartmentation of excitation energy in the antenna, (ii) it is the site of a conformational change in the first second of illumination, and (iii) it is involved in the mechanisms of nonphotochemical quenching (NPQ). On the basis of the multi-frequency PMF analysis, we reconciled two apparently antagonistic aspects of chlorophyll a fluorescence in vivo: it is heterogeneous with respect to the kinetic structure (several lifetime components) and homogeneous with respect to ...Continue Reading

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Citations

Aug 1, 2007·Photosynthesis Research·George C PapageorgiouKostas Stamatakis
Jul 20, 2012·Photosynthesis Research·Alexandrina Stirbet, Govindjee
Mar 5, 2013·Photosynthesis Research·Gert SchanskerGyőző Garab
Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
Jul 31, 2013·Biochimica Et Biophysica Acta·Thomas de MarchinFabrice Franck
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lloyd Donaldson

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