PMID: 6159004Nov 4, 1980Paper

Correlation between phagocytic and membrane surface properties reflected by partitioning of human peripheral blood monocytes in two-polymer aqueous phases

Biochimica Et Biophysica Acta
H WalterM Hill

Abstract

Human peripheral blood monocyte-enriched fractions (identified by staining for peroxidase and by sizing) were obtained by velocity sedimentation at unit gravity of peripheral blood mononuclear cells. They were then fractionated by countercurrent distribution (a multiple-extraction procedure) in a charged Dextran/poly(ethylene glycol) aqueous phase system. The monocytes remained viable after the separation (order of 90%). Cells obtained from different cavities along the extraction train were tested for their ability to phagocytize latex particles. With increasing partition coefficient (presumably higher charge-associated membrane properties) the ratio of monocytes that phagocytized to monocytes that did not phagocytize increased appreciably. When, however, monocytes were permitted to phagocytize particles prior to countercurrent distribution, an increase in partition coefficient was associated with an appreciable decrease in the above-specified ratio. Control experiments indicate that the observed change in partitioning behavior cannot be ascribed to an alteration in size and/or density of the monocytes as a function of phagocytosis. It may be due to the internalization of charged surface groups during phagocytosis. We conclude ...Continue Reading

Citations

Oct 22, 2008·Biomedical Microdevices·Jeffrey R Soohoo, Glenn M Walker
Mar 1, 1990·Veterinary Immunology and Immunopathology·A SeidelG Polzer
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Kim LangfelderAxel A Brakhage
May 19, 2000·Infection and Immunity·R Romero-MartinezH Torres-Guerrero
Apr 4, 2021·Journal of Fungi·Sichen LiuJoshua D Nosanchuk

Related Concepts

Plasma Membrane
Cell Separation
Countercurrent Chromatography
Saviosol
Monocytes
Phagocytosis
Vigilon
Surface Properties

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