PMID: 6973997Apr 15, 1981Paper

Correlation between preincubation time of the leukocyte feeder-layer and in vitro granulocytic-macrophage colony growth

Bollettino della Società italiana di biologia sperimentale
A RolandiA Abbadessa

Abstract

Granulocytic-macrophage colony in vitro formation depends on the presence of the colony stimulating factor (CSF). When, in double layer agar method, the CSF is provided from leucocyte underlayers, the pre-incubation time of the feeder-layers must be kept constant. The results obtained in this study have demonstrated in fact that the growth of the colonies can be remarkably different if feeder-layers are incubated for 1, 7 or 14 days.

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