Correlation between production of acyl homoserine lactones and proteases in an Aeromonas hydrophila aroA live vaccine

Veterinary Microbiology
José VivasA J Villena

Abstract

Aeromonas hydrophila is a pathogen that causes disease in a wide range of homeothermic and poikilothermic hosts due to its multifactorial virulence. We have previously described the characterisation and use of an auxotrophic aroA mutant of the A. hydrophila AG2 strain as a live attenuated vaccine against A. hydrophila infections in rainbow trout (Oncorhynchus mykiss). In this study we report the expression of extracellular proteolytic activities and of quorum-sensing molecules by this mutant grown under different culture conditions, and in vaccine inocula. The aroA strain expresses extracellular proteases efficiently during in vitro growth and this ability is retained in vaccine inocula that were prepared by washing the bacterial cultures and resuspending the cells in phosphate-buffered saline. Since proteases are considered to be major bacterial antigens, the expression of these enzymes in the live attenuated vaccine may contribute to the superior protection afforded by these kind of vaccines. On the other hand, the production of serine- and metalloprotease activities in A. hydrophila has been described as controlled in a cell density-dependent fashion, through a mechanism known as quorum sensing. A microtiter method was devel...Continue Reading

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Citations

Oct 24, 2007·BMC Microbiology·Kamlesh JangidYogesh S Shouche
Mar 10, 2015·Applied and Environmental Microbiology·Sara Remuzgo-MartínezJosé Ramos-Vivas
Jun 18, 2009·Chembiochem : a European Journal of Chemical Biology·Verena ThielStefan Schulz
Sep 11, 2019·Journal of Applied Microbiology·A M LabellaJ J Borrego

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