Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil

Experimental & Applied Acarology
Kauê Rodriguez MartinsRenato Andreotti

Abstract

The tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain...Continue Reading

References

May 1, 1972·Australian Veterinary Journal·D F Mahoney, D R Ross
Feb 1, 1973·Journal of Economic Entomology·R O DrummondO H Graham
Aug 1, 1993·Journal of Clinical Microbiology·I S EriksG H Palmer
Dec 1, 1994·Parasite : Journal De La Société Française De Parasitologie·D H AguirreA A Guglielmone
Oct 23, 1997·Clinical Microbiology Reviews·D Raoult, V Roux
Feb 27, 1999·International Journal for Parasitology·J E Frisch
Feb 24, 2001·Annals of the New York Academy of Sciences·R D SmithA Nari
Aug 2, 2002·Veterinary Microbiology·José de la FuenteKatherine M Kocan
Oct 15, 2003·Clinical Microbiology Reviews·Katherine M KocanRoy D Meléndez
Dec 18, 2004·Annals of the New York Academy of Sciences·G M de AndradeO Vidotto
Nov 23, 2005·Journal of Virological Methods·Guan M KeHung J Liu
Mar 27, 2007·The Veterinary Clinics of North America. Food Animal Practice·Jeffery A Carroll, Neil E Forsberg
Nov 3, 2010·Transboundary and Emerging Diseases·P Aubry, D W Geale
Apr 29, 2011·Journal of Dairy Science·L E HulbertM A Ballou
Aug 24, 2013·Frontiers in Cellular and Infection Microbiology·Mária Kazimírová, Iveta Štibrániová
Jul 24, 2014·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Laerte GrisiHumberto Silva Villela
Nov 18, 2015·Parasites & Vectors·Cecília José VeríssimoIsabel Kinney Ferreira de Miranda Santos
Sep 10, 2016·BioMed Research International·Rosa Estela Quiroz-CastañedaSergio Darío Rodríguez-Camarillo
Jan 17, 2017·Ticks and Tick-borne Diseases·Rodrigo GigliotiMárcia Cristina de Sena Oliveira
Apr 14, 2018·PLoS Neglected Tropical Diseases·Jan PernerJosé M C Ribeiro
Apr 2, 2019·Ticks and Tick-borne Diseases·Inalda Angélica de Souza RamosMarcos Rogério André

❮ Previous
Next ❯

Citations

Jul 24, 2020·Experimental & Applied Acarology·Michael LightN Kirk Hillier

❮ Previous
Next ❯

Methods Mentioned

BETA
prophylactic treatment
PCR
ELISA

Software Mentioned

StepOne
primer3

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.