PMID: 2480406Jul 1, 1989Paper

Correlation between RIA-EIA-ELISA methods for alpha-fetoprotein research.

The Journal of Nuclear Medicine and Allied Sciences
M Pastore, S Francioni

Abstract

To-day, the possibility of researching alpha-fetoprotein is given not only by the RIA method but also some immunoenzymatic methods. The Authors have compared three types of methods: RIA, EIA and ELISA, and have also studied the correlation coefficient in 30 samples. Method in solid phase (coated-tubes) and mouse monoclonal antibodies are used for the radioimmunologic method. The ELISA method utilizes two monoclonal antibodies: the former is coated to the walls of the test tubes (mouse monoclonal antibodies) and the latter is labelled with bovine alkaline phosphatase. The EIA methods is a solid phase enzyme immunoassay based on the sandwich principle. It consists of beads coated of goat antibodies and goat anti-AFP antibodies conjugated with horseradish peroxidase. The correlation coefficient is: RIA-ELISA = r 0.95; RIA-EIA = r 0.93; EIA-ELISA = r 0.96. The correlation coefficient from both the RIA method (taken as a reference) and the immunoenzymatic methods is good, so that the passage from RIA to enzymatic methods is possible without problems of under or above dosage of alpha-fetoprotein values.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.