Correlation between skeletal muscle fiber type and free amino acid levels in Japanese Black steers

Animal Science Journal = Nihon Chikusan Gakkaihō
Daisuke MashimaWataru Mizunoya

Abstract

Free amino acids are important components of tastants and flavor precursors in meat. To clarify the correlation between muscle fiber type and free amino acids, we determined the concentrations of various free amino acids and dipeptides in samples of different muscle tissues (n = 21), collected from 26-month-old Japanese Black steers (n = 3) at 2 days postmortem. The proportions of the myosin heavy chain (MyHC), slow (MyHC1) and fast (MyHC2) isoforms were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The contents of free amino acids and dipeptides were measured by high performance liquid chromatography (HPLC). The MyHC isoform composition varied among the tissue samples. The MyHC1 proportion ranged from 6.9% ± 3.9% to 83.3% ± 16.7%. We confirmed that there was a strong positive correlation between MyHC1 composition and total free amino acid concentrations, including those for two dipeptides. Among the 31 measured free amino acids and dipeptides, 11 showed significant positive correlations and five showed significant negative correlations with MyHC1 composition. These results suggest that a high MyHC1 content induces high free amino acid contents in bovine muscles possibly because of greater ...Continue Reading

References

Aug 1, 1979·The American Journal of Clinical Nutrition·S S SchiffmanD Reilly
May 1, 1970·Comparative Biochemistry and Physiology·K G Crush
Jul 1, 1981·Physiology & Behavior·S S SchiffmanJ Gagnon
Sep 22, 2000·Microscopy Research and Technique·D Pette, R S Staron
Mar 15, 2002·Nature·Greg NelsonCharles S Zuker
Dec 5, 2002·Journal of Animal Science·K S KirchoferB L Gwartney
Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Luis A Rubio
Aug 19, 2007·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Wendy W H ZhongJoseph F Y Hoh

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Citations

Jul 22, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Yusuke KomiyaKeizo Arihara

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Methods Mentioned

BETA
Protein Assay
electrophoresis

Software Mentioned

ImageJ
Excel

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