Correlation between TAMs and proliferation and invasion of type I endometrial carcinoma

Asian Pacific Journal of Tropical Medicine
Hong-Lin HuHai-Xia Pan

Abstract

To explore the correlation between tumor-associated macrophages and the proliferation and invasion of type I endometrial carcinoma. Immunohistochemistry was used to investigate the infiltration of macrophages in normal and different types of hyperplastic endometrial lesions. The proliferation and invasion ability of type I endometrial carcinoma cell line RL95-2 influenced by mononuclear macrophage cell line THP-1 (constructed M2 type macrophages) was detected by CCK8 and transwell technologies respectively. Transwell was used to evaluate the recruiting ability of RL95-2 on THP-1 cells. Otherwise, the western blot was also used to detect the expression of CyclinD1 and MMP-2 in RL95-2 with the influence of THP-1. Immunohistochemistry result showed a positive correlation between the number of infiltrating macrophages and the progression of endometrial hyperplasia. THP-1 recruited by RL95-2 could promote its proliferation and invasion and enhance the expression of the CyclinD1 and MMP-2 protein in a time dependent manner (P < 0.05). Increase of the number of infiltrating macrophages and its contribution to the tumor inflammatory microenvironment may result in the development of the type I endometrial carcinoma.

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Citations

Jun 18, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·A V KubyshkinO V Karapetian
Nov 20, 2020·Bulletin of Mathematical Biology·Szabolcs SuvegesDumitru Trucu
Nov 17, 2021·Journal of Zhejiang University. Science. B·Yi ZhaoHelin Feng

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