PMID: 2489891Jan 1, 1989Paper

Correlation between the illumination time and cytotoxicity of light-cured composite resins

Shōni shikagaku zasshi. The Japanese journal of pedodontics
Y ItoH Oguchi

Abstract

This study was undertaken to compare the relative cytotoxicity of light cured composite resins, and the cytotoxic reduction of light cured composite resins by illumination time by means of in vitro cell culture test. Light cured composite resins (P-30, Silux, Occlusin, Graft LC, Litefil A, Photo Clearfil A, Palfique light and Herculite XR) were filled into glass tubes (4 mm inner dia. x 1 mm high), then illuminated for 0, 10, 20, 30, 40, 50 and 60 seconds through a matrix strip with direct contact ot an Optilux light source (3M), just before testing. Fibroblasts, 2 ml of 1 x 10(5) cells/ml in Eagle's MEM medium supplemented with 10% FCS, penicillin (100 U/ml) and streptomycin (100 micrograms/ml) were evenly seeded on a cover glass in a tissue culture dish and incubated for 24 hours at 37 degrees C in a humidified atmosphere of 5% CO2 in the air. After 24 hours of incubation, the medium was replaced with 2 ml of agar overlay medium consisting of 10% FCS, Eagle's MEM medium and 1.5% agar. Glass tubes (N = 8) filled with the light cured composite resins were placed at the center of the agar, and incubated for 24 hours in 5% CO2 in air at 37 degrees C. After 24 hours of incubation, the cover glass was removed and the cells were fix...Continue Reading

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