Correlation between the levels of miR-21, miR-34c, miR-140 and miR-375 in the sperm from in vitro fertilization patients and the embryo quality

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Wen Jiang, Nenghui Liu

Abstract

To explore the correlation between sperm-originated miRNAs and embryo quality by detecting the expression levels of miR-21, miR-34c, miR-140 and miR-375 in the sperm from in vitro fertilization (IVF) patients. The fresh semen specimens were collected from 44 male patients who received the IVF cycle in the Xiangya Hospital Reproductive Center from September to December in 2012. The expression levels of miR-34c, miR-140, miR-21 and miR-375 were detected by real-time fluorescent quantitative PCR. The embryos on day 2 and day 3 after fertilization were divided into the experimental and the control group, with an average embryo scores less or greater than 8, respectively. Then we compared the general and experimental data between the 2 groups respectively and analyzed the correlation between the miRNAs levels in sperm and embryo quality. The expression of miR-34c, miR-140, miR-21 and miR-375 in sperms from the experimental group was significantly lower than that in the control group (P<0.05). There was no significant difference in retrieved follicles, metaphase II stage ovocytes, fertilized oocytes, cleavage number and fertilization rate between the experimental group and the control group (P>0.05), while the cleavage rate on day 2 ...Continue Reading

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