Correlation of antigen-specific IFN-γ responses of fresh blood samples from Mycobacterium avium subsp. paratuberculosis infected heifers with responses of day-old samples co-cultured with IL-12 or anti-IL-10 antibodies

Veterinary Immunology and Immunopathology
Heidi MikkelsenG Jungersen

Abstract

Paratuberculosis is a chronic infection of the intestine of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Early stage MAP infection can be detected by measuring cell-mediated immune responses using the interferon gamma (IFN-γ) assay. Whole blood samples are cultured overnight with specific MAP antigens followed by quantification of IFN-γ by ELISA. It is recommended that the time interval from sampling to culture does not exceed eight hours but addition of the co-stimulating cytokine interleukin 12 (IL-12) or anti-IL-10 antibodies to culture have been demonstrated to enhance IFN-γ responses of cultures stimulated with Johnin purified protein derivative (PPDj). Here we examined the correlation of IFN-γ production in response to PPDj and 15 recombinant antigens in day-old blood samples from heifers 10-21 months of age from a MAP infected herd with addition of either recombinant bovine IL-12 or anti-bovine IL-10 antibody with IFN-γ production in sample day samples. IFN-γ responses of sample day samples showed high correlation with responses to some antigens in day-old samples with addition of IL-12 or anti-IL-10 antibodies to cultures, indicating that day-old protocols can be applied as an alternative to th...Continue Reading

References

Dec 19, 2000·Veterinary Microbiology·J R Stabel
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·G JungersenP Lind
Jan 1, 1950·Cancer·W J YOUDEN
May 20, 2005·American Journal of Veterinary Research·Douglas J WeissMitchell S Abrahamsen
Sep 9, 2006·Clinical and Vaccine Immunology : CVI·Makeda SemretMarcel A Behr
Sep 27, 2008·Preventive Veterinary Medicine·Søren Saxmose Nielsen, Nils Toft
Nov 26, 2008·Veterinary Immunology and Immunopathology·Heidi MikkelsenSøren Saxmose Nielsen
Sep 19, 2009·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Patricia Méndez-Samperio
Feb 22, 2011·Veterinary Microbiology·Søren Saxmose NielsenIan Andrew Gardner
Apr 13, 2011·Veterinary Microbiology·Heidi MikkelsenGregers Jungersen
May 28, 2011·Veterinary Immunology and Immunopathology·Gregers JungersenSusanne N Grell

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