Correlation of cyclic AMP accumulation and relaxant actions of salmeterol and salbutamol in bovine tracheal smooth muscle

British Journal of Pharmacology
K E EllisR A Challiss

Abstract

1. The ability of salmeterol to stimulate cyclic AMP accumulation and relaxation has been compared with that of salbutamol in bovine tracheal smooth muscle. In addition, the anti-spasmogenic effects of these agents and their abilities to modulate histamine-stimulated [3H]-inositol phosphate accumulation have also been investigated. 2. In tissue strips, a close temporal correlation was found to exist between salmeterol (0.1 microM)-induced relaxation of methacholine (500 nM)-induced tone and cyclic AMP accumulation, both maximal reversal of induced tone (26.2 +/- 6.0%) and maximal levels of cyclic AMP accumulation being achieved after 30-40 min. In contrast to salmeterol, salbutamol exerted greater and more rapid effects on both parameters. Maximal reversal of methacholine-induced tone (79.3 +/- 14.0%) and maximal levels of cyclic AMP accumulation were produced within 5 min. 3. Salmeterol-induced cyclic AMP accumulation (EC50 = 5.3 [1.8 - 15.2] nM) and inhibition of histamine (0.1 mM)-stimulated [3H]-inositol phosphate accumulation (IC50 = 1.4 [0.3-6.3] nM) were both more potent than those induced by salbutamol (EC50 = 169 [99 - 290] nM; IC50 = 13.8 [7.0 - 27.4] nM). However, maximal effects exerted by each of these agents were ...Continue Reading

References

Jan 1, 1991·Pharmacology & Therapeutics·A Levitzki, A Bar-Sinai
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A DeegN D Goldberg
Nov 1, 1993·British Journal of Pharmacology·A T NialsC J Vardey
Mar 1, 1994·The European Respiratory Journal·G P AndersonK F Rabe
Apr 1, 1994·Pharmacology & Therapeutics·E R ChilversR A Challiss

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Citations

Oct 6, 2001·American Journal of Respiratory and Critical Care Medicine·J C Kips, R A Pauwels
Sep 6, 2013·Respiratory Research·Kentaro MizutaCharles W Emala
Apr 29, 1998·Chest·R H MooreB F Dickey
May 18, 2004·European Journal of Pharmacology·Katsuo KoikeYoshio Tanaka
May 14, 2004·Journal of Biomolecular Screening·Yuji M MishinaCraig M Thompson
May 23, 2012·Pharmacological Reviews·Mario CazzolaM Gabriella Matera

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