PMID: 9428343Jan 15, 1998Paper

Correlation of expression of NCAM and GD3 ganglioside to motile behaviour in neoplastic glia

Anticancer Research
A GratsaG J Pilkington

Abstract

Studies of developing mammalian tissues have established that certain neural cell adhesion molecules (NCAMs) may be down-regulated during the migratory phase concurrent with an increase in levels of matrix metalloproteinases. In addition, there is evidence that simple gangliosides such as GD3 are transiently present on the surface of such migratory cells. Since migration, or motility, is a prerequisite for diffuse local invasion of brain by neoplastic cells, the expression of NCAM and GD3 on brain tumour cells was studied in order to establish their possible role in the invasive process. An astrocytoma parent cell line (IPSB-18) and two morphologically distinct, cloned cell lines (clone 1 and 12) derived from it, were used in in vitro motility assays using 8 microns porosity polycarbonate filters in "Transwell" modified Boyden chambers. Immunocytochemical staining with anti-NCAM monoclonal antibodies (UJ13A and ERIC-1) and with the anti-ganglioside monoclonal antibodies LB1 (which recognises GD3) and A2B5 (which recognises a range of simple gangliosides) showed that some cells in culture from the parent line were positive for either NCAM or GD3; clone 1 was NCAM positive but GD3 negative, while clone 12 was NCAM negative but ga...Continue Reading

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.